Leupen, Sarah M.Tobet, Stuart A.Crowley, W. F. Jr.,Kaila, Kai2020-06-222020-06-222003-07-01Sarah M. Leupen, Stuart A. Tobet, W. F. Crowley, Jr., Kai Kaila, Heterogeneous Expression of the Potassium-Chloride Cotransporter KCC₂ in Gonadotropin-Releasing Hormone Neurons of the Adult Mouse, Endocrinology, Volume 144, Issue 7, 1 July 2003, Pages 3031–3036, https://doi.org/10.1210/en.2002-220995https://doi.org/10.1210/en.2002-220995http://hdl.handle.net/11603/18953In mature central neurons, chloride extrusion mediated by the K-Cl cotransporter KCC₂ appears to be largely responsible for the Cl− driving force that allows γ-aminobutyric acidA (GABAA) receptor activation to trigger a hyperpolarization. In its absence, GABA’s effect is typically depolarizing and often excitatory. We examined the colocalization of KCC₂ and GnRH in adult male and female mice using a combined in situ hybridization-immunofluorescence procedure. We found that KCC₂ was localized to approximately 34% of GnRH neurons. This proportion was similar in females and males. However, females exhibited a marked rostrocaudal gradient of colocalization that was not seen in males. By contrast, KCC₂ was localized to nearly all vasopressin neurons of the supraoptic nucleus. These results indicate that a substantial fraction of GnRH neurons may be depolarized and excited by GABAA receptor activation throughout life, supporting the existence of functionally heterogeneous subpopulations.6 pagesen-USThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.NeuroendocrinologyHeterogeneous Expression of the Potassium-Chloride Cotransporter KCC₂ in Gonadotropinheterogeneous subpopulationsγ-aminobutyric acidᴀ (GᴀBAᴀ)Text