Borhani, ShayanGurramkonda, ChandrasekharFrey, DouglasTolosa, LeahSwartz, JamesRao, Govind2020-11-192020-11-19Shayan Borhani et al., Developments in Insulin Manufacturing Using Cell-Free Systems, https://elrig.org/downloads/ddd/3040_Developments_in_Insulin_Manufacturing_Using_Cell_Free_Systems.pdfhttp://hdl.handle.net/11603/20102The primary aim of this method is to emulate the naturally occurring mechanisms which take place in vivo, when converting preproinsulin into metabolically active insulin (4). Figure 2, displays the binding of pre-proinsulin to a CM HiTrap FF column (pH 3.0) followed by cleavage and threonine addition to the terminal B29 residue. The final product is then eluted using a linear salt gradient.1 pageen-USThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.insulincell free systemsdiabetesText