Schreier, Harold J.Dejtisakdi, WipaweeEscalante, Jose OrtegaBrailo, Marina2019-03-222019-03-222012-10Harold J. Schreier, Wipawee Dejtisakdi, Jose Ortega Escalante, and Marina Brailo, Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant, Appl Environ Microbiol. 2012 Oct; 78(19): 7120–7123, DOI: 10.1128/AEM.01794-12https://doi.org/10.1128/AEM.01794-12http://hdl.handle.net/11603/13127A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.4 pagesen-USThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.Planctomyces limnophilusEZ-Tn5 transposomephosphoenolpyruvate carboxykinaseText