Zhang, YongxiaAgreda, PatriciaKelley, ShannonGaydos, CharlotteGeddes, Chris2024-10-012024-10-012011-03Zhang, Yongxia, Patricia Agreda, Shannon Kelley, Charlotte Gaydos, and Chris D. Geddes. “Development of a Microwave—Accelerated Metal-Enhanced Fluorescence 40 Second, <100 Cfu/mL Point of Care Assay for the Detection of Chlamydia Trachomatis.” IEEE Transactions on Biomedical Engineering 58, no. 3 (March 2011): 781–84. https://doi.org/10.1109/TBME.2010.2066275.https://doi.org/10.1109/TBME.2010.2066275http://hdl.handle.net/11603/36588An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with “bow-tie” structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new “ release and detect” platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.11 pagesen-US© 2011 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.GoldDNAChlamydia trachomatis (CT)AccelerationlysisMicrowave technologyTestingMicroorganismsElectromagnetic heatingMicrowave theory and techniquesFluorescencemicrowave-accelerated metal-enhanced fluorescence (MAMEF)DiseasesText