Vo-Dinh, TuanAlarie, Jean-PierreCullum, BrianGriffin, Guy D.2024-09-042024-09-042000-07Vo-Dinh, Tuan, Jean-Pierre Alarie, Brian M. Cullum, and Guy D. Griffin. “Antibody-Based Nanoprobe for Measurement of a Fluorescent Analyte in a Single Cell.” Nature Biotechnology 18, no. 7 (July 2000): 764–67. https://doi.org/10.1038/77337.https://doi.org/10.1038/77337http://hdl.handle.net/11603/36056We report here the application of an antibody-based nanoprobe for in situ measurements of a single cell. The nanoprobe employs antibody-based receptors targeted to a fluorescent analyte, benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyrene (BaP) and of the BaP–DNA adduct. Detection of BPT is of great biomedical interest, since this species can serve as a biomarker for monitoring DNA damage due to BaP exposure and for possible precancer diagnosis. The measurements were performed on the rat liver epithelial clone 9 cell line, which was used as the model cell system. Before making measurements, the cells were treated with BPT. Nanoprobes were inserted into individual cells, incubated 5 min to allow antigen–antibody binding, and then removed for fluorescence detection. We determined a concentration of 9.6 ± 0.2 × 10⁻¹¹ M for BPT in the individual cells investigated. The results demonstrate the possibility of in situ measurements inside a single cell using an antibody-based nanoprobe.4 pagesen-USThis work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.Public Domainhttps://creativecommons.org/publicdomain/mark/1.0/BiomedicineBiomedical Engineering/BiotechnologyLife SciencesBioinformaticsAgriculturegeneralBiotechnologyText