Zengel, J. M.Lindahl, L.2019-06-042019-06-041981-01J M Zengel and L Lindahl, High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli, J Bacteriol. 1981 Jan; 145(1): 459–465, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC217294/http://hdl.handle.net/11603/14011We have constructed a high-copy-number plasmid carrying an allele of the supD gene (supD43,74). The plasmid conferred temperature-sensitive suppression of amber mutations. Strains carrying the plasmid exhibited 50 to 60% suppression at 30 degrees C but little or no suppression at 42 degrees C. After a temperature shift from 30 to 42 degrees C the efficiency of suppression decreased gradually over a 60- to 90-min period before reaching the 42 degrees C steady-state level of suppression.7 pagesen-USThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.amber mutationsEscherichia colitemperature-sensitiveText