Cullum, BrianGriffin, Guy D.Vo-Dinh, Tuan2024-09-042024-09-042002-05-13Cullum, Brian M., Guy D. Griffin, and Tuan Vo-Dinh. “Nanosensors: Design and Application to Site-Specific Cellular Analyses.” In Biomedical Diagnostic, Guidance, and Surgical-Assist Systems IV, 4615. (May 13, 2002):148–54. https://doi.org/10.1117/12.466642.https://doi.org/10.1117/12.466642http://hdl.handle.net/11603/35966International Symposium on Biomedical Optics, 2002, San Jose, CA, United StatesWe report the development and application of an antibody-based nanoprobe for in situ measurements within a single cell. The nanoprobe has an antibody-based probe targeted to benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyrene (BaP) and the BaP-DNA adduct. Detection of BPT is of biomedical interest since this species can potentially serve as a biomarker for the monitoring of DNA damage due to BaP exposure and for possible pre-cancer diagnoses. The measurements were performed on the rat liver epithelial Clone 9 cell line, which was used as the model cell system. Nanoprobes were inserted into individual cells, incubated five minutes to allow antigen-antibody binding, and then removed for fluorescence detection. Prior to measurements, the cells had been treated with BPT. A concentration of 9.6 +/- 0.2 x 10⁻¹¹ M has been determined for BPT in the individual cells investigated. The results demonstrate the possibility of in situ measurements inside a single cell using an antibody-based nanoprobe.7 pagesen-USTHIS WORK WAS WRITTEN AS PART OF ONE OF THE AUTHOR'S OFFICIAL DUTIES AS AN EMPLOYEE OF THE UNITED STATES GOVERNMENT AND IS THEREFORE A WORK OF THE UNITED STATES GOVERNMENT. IN ACCORDANCE WITH 17 U.S.C. 105, NO COPYRIGHT PROTECTION IS AVAILABLE FOR SUCH WORKS UNDER U.S. LAW.PUBLIC DOMAINhttps://creativecommons.org/publicdomain/mark/1.0/Text