Eggleton, C.D.Gupta, V.K.2018-10-222018-10-222012-06-15V.K. Gupta ,C.D. Eggleton, A Theoretical Method to determine unstressed off-rate from multiple bond force spectroscopy, Colloids Surf B Biointerfaces. 2012 June 15; 95: 50–56. doi:10.1016/j.colsurfb.2012.02.01010.1016/j.colsurfb.2012.02.010http://hdl.handle.net/11603/11624Using dynamic force spectroscopy to measure the kinetic off-rates of intermolecular bonds currently requires the isolation of single molecules. This requirement arises in part because no tractable analytic method for determining kinetic off-rates from the rupture of a large number of bonds under dynamic forces is currently available. We introduce a novel method for determining the unstressed off-rate from dynamic force spectroscopy experiments involving a large number of bonds. Using both the Bell and Dembo models we show that the unstressed off-rate calculated using the proposed method is in good agreement with the prescribed unstressed off-rate used in Monte-Carlo simulations of multiple bond dynamic force spectroscopy experiments given initial number of bonds (50–500) and loading rate 10³ – 10⁶ pN/s.23 pagesen-USThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.Attribution-NonCommercial-NoDerivs 3.0 United Stateshttp://creativecommons.org/licenses/by-nc-nd/3.0/us/kinetic off-ratesmultiple bond forcespectroscopyMonte-Carlo simulationsUMBC High Performance Computing Facility (HPCF)Text