H. J., SchreierC. A., RostkowskiE. M., Kellner2019-03-152019-03-151993-02H J Schreier, C A Rostkowski, E M Kelln, Altered regulation of the glnRA operon in a Bacillus subtilis mutant that produces methionine sulfoximine-tolerant glutamine synthetase, J Bacteriol. 1993 Feb;175(3):892-7, DOI: 10.1128/jb.175.3.892-897.1993https://doi.org/10.1128/jb.175.3.892-897.1993http://hdl.handle.net/11603/13075A Bacillus subfilis mutant that produced glutamine synthetase (GS) with altered sensitivity to DL-methionine sulfoximine was isolated. The mutation, designated glnA33, was due to a T- A-to-C- G transition, changing valine to alanine at codon 190 within the active-site C domain. Altered regulation was observed for GS activity and antigen and mRNA levels in a B. subtilis glnA33 strain. The mutant enzyme was 28-fold less sensitive to DL-methionine sulfoximine and had a 13.0-fold-higher Km for hydroxylamine and a 4.8-fold-higher Km for glutamate than wild-type GS did.6 pagesen-USThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.Bacillus subfilisglutamine synthetase (GS)DL-methionine sulfoximineLmethionine-SR-sulfoximine (MSX)Text