Dassarma, PriyaKaran, RamKim, Jong-MyoungPecher, Wolf TDassarma, Shiladitya2017-06-232017-06-232016DasSarma, P., Karan, R., Kim, J.-M., Pecher, W., & DasSarma, S. (January 01, 2016). Bioengineering Novel Floating Nanoparticles for Protein and Drug Delivery. Materials Today: Proceedings, 3, 2, 206-210.10.1016/j.matpr.2016.01.058http://hdl.handle.net/11603/4186Gas vesicle nanoparticles (GVNPs) are hollow protein nanoparticles produced by Halobacterium sp. NRC-1 which are being engineered for protein delivery. To advance the bioengineering potential of GVNPs, a strain of NRC-1 deleted for the gvpC gene (ΔgvpC) was constructed and a synthetic gene coding for Gaussia princeps luciferase was fused to an abbreviated gvpC gene on an expression plasmid. When introduced into theΔgvpC strain, an active GvpC-luciferase fusion protein bound to GVNPs resulted. These results represent both a technical improvement in the GVNP display system and its expansion for the display of active enzymes.8 pagesen-USGas vesicle nanoparticles (GVNPs)vaccineluciferaseGaussia princepsenzyme displayText