Eisenmann, David MAbete, Patricia2021-01-292021-01-292018-01-0111925http://hdl.handle.net/11603/20640The precise temporal control of gene expression is an essential aspect of metazoan development. The ecdysozan C. elegans goes through six life stages (embryo, L1 to L4, and adult) separated by molts, and analyses of modENCODE data showed that most members of the large cuticle collagen (col) gene family express predominantly at a single stage. This temporal preference motivated us to investigate the mechanisms underlying col gene expression and here we focus on a subset of col genes expressed in the L4 stage. We identified minimal promoter regions of <300 bp for col-38, col-49, and col-63 in which we predicted cis-regulatory sequences and evaluated their function in vivo via mutagenesis of a col-38papplication:pdfText