The problem of genetic code misreading during protein synthesis
Loading...
Links to Files
Author/Creator
Author/Creator ORCID
Date
2018-12-17
Type of Work
Department
Program
Citation of Original Publication
Kartikeya Joshi , Ling Cao, Philip J. Farabaugh, The problem of genetic code misreading during protein synthesis, Yeast, Volume 36, Issue 1, 2018, https://doi.org/10.1002/yea.3374
Rights
This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
This is the peer reviewed version of the following article: Kartikeya Joshi , Ling Cao, Philip J. Farabaugh, The problem of genetic code misreading during protein synthesis, Yeast, Volume 36, Issue 1, 2018, https://doi.org/10.1002/yea.3374, which has been published in final form at https://onlinelibrary.wiley.com/doi/epdf/10.1002/yea.3374. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Access to this item will begin on December 17. 2019
This is the peer reviewed version of the following article: Kartikeya Joshi , Ling Cao, Philip J. Farabaugh, The problem of genetic code misreading during protein synthesis, Yeast, Volume 36, Issue 1, 2018, https://doi.org/10.1002/yea.3374, which has been published in final form at https://onlinelibrary.wiley.com/doi/epdf/10.1002/yea.3374. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Access to this item will begin on December 17. 2019
Abstract
Saccharomyces cerevisiae has been an important model for determining the frequency
of translational misreading events, those in which a tRNA pairs incorrectly to the
mRNA and inserts an amino acid not specified by the codon in the mRNA. Misreading
errors have been quantified in vivo using reporter protein systems or mass spectrom-
etry with both approaches converging on a simple model for most misreading. The
available data show that misreading tRNAs must form stereotypical base mismatches
that correspond to those that can mimic Watson–Crick base pairs when formed in the
ribosomal A site. Errors involving other mismatches occur significantly less frequently.
This work debunks the idea of an average misreading frequency of 5 × 10
−4
per codon
that extends across the genetic code. Instead, errors come in two distinct classes—
high frequency and low frequency events—with most errors being of the low
frequency type. A comparison of misreading errors in S. cerevisiae and Escherichia coli
suggests the existence of a mechanism that reduces misreading frequency in yeast;
this mechanism may operate in eukaryotes generally.