Browsing by Subject "measuring protein turnover"
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Item Measurement of Fractional Synthetic Rates of Multiple Protein Analytes by Triple Quadrupole Mass Spectrometry(Oxford University Press, 2012-03-01) Lee, Anita Y H; Yates, Nathan A; Ichetovkin, Marina; Deyanova, Ekaterina; Southwick, Katie; Fisher, Timothy S; Wang, Weixun; Loderstedt, James; Walker, Nykia; Zhou, Haihong; Zhao, Xuemei; Sparrow, Carl P; Hubbard, Brian K; Rader, Daniel J; Sitlani, Ayesha; Millar, John S; Hendrickson, Ronald CBACKGROUND Current approaches to measure protein turnover that use stable isotope-labeled tracers via GC-MS are limited to a small number of relatively abundant proteins. We developed a multiplexed liquid chromatography–selected reaction monitoring mass spectrometry (LC-SRM) assay to measure protein turnover and compared the fractional synthetic rates (FSRs) for 2 proteins, VLDL apolipoprotein B100 (VLDL apoB100) and HDL apoA-I, measured by both methods. We applied this technique to other proteins for which kinetics are not readily measured with GC-MS. METHODS Subjects were given a primed-constant infusion of [5,5,5-D₃]-leucine (D₃-leucine) for 15 h with blood samples collected at selected time points. Apolipoproteins isolated by SDS-PAGE from lipoprotein fractions were analyzed by GC-MS or an LC-SRM assay designed to measure the M+3/M+0 ratio at >1% D₃-leucine incorporation. We calculated the FSR for each apolipoprotein by curve fitting the tracer incorporation data from each subject. RESULTS The LC-SRM method was linear over the range of tracer enrichment values tested and highly correlated with GC-MS (R² > 0.9). The FSRs determined from both methods were similar for HDL apoA-I and VLDL apoB100. We were able to apply the LC-SRM approach to determine the tracer enrichment of multiple proteins from a single sample as well as proteins isolated from plasma after immunoprecipitation. CONCLUSIONS The LC-SRM method provides a new technique for measuring the enrichment of proteins labeled with stable isotopes. LC-SRM is amenable to a multiplexed format to provide a relatively rapid and inexpensive means to measure turnover of multiple proteins simultaneously.