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    The FixK2 protein is involved in regulation of symbiotic hydrogenase expression in Bradyrhizobium japonicum

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    http://hdl.handle.net/11603/10847
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    Author/Creator
    Durmowicz, Meredith C.
    Maier, Robert J.
    Date
    1998-06
    Type of Work
    4 pages
    Text
    journal articles
    Department
    Beverly K. Fine School of the Sciences
    Citation of Original Publication
    Durmowicz, M. C., & Maier, R. J. (1998). The FixK2 protein is involved in regulation of symbiotic hydrogenase expression in Bradyrhizobium japonicum. Journal Of Bacteriology, 180(12), 3253-3256.
    Subjects
    Genes, Bacterial
    Bacterial Proteins/*metabolism
    Hydrogenase/*genetics
    Rhizobiaceae/*genetics
    Rhizobiaceae/*metabolism
    Bacterial Proteins/genetics
    Base Sequence
    Binding Sites/genetics
    DNA, Bacterial/genetics
    Gene Expression Regulation, Bacterial
    Gene Expression Regulation, Enzymologic
    Molecular Sequence Data
    Mutation
    Nitrogen Fixation/genetics
    Phenotype
    Promoter Regions, Genetic
    Symbiosis
    Transcriptional Activation
    Abstract
    The roles of the nitrogen fixation regulatory proteins NifA, FixK1, and FixK2 in the symbiotic regulation of hydrogenase structural gene expression in Bradyrhizobium japonicum have been investigated. Bacteroids from FixJ and FixK2 mutants have little or no hydrogenase activity, and extracts from these mutant bacteroids contain no hydrogenase protein. Bacteroids from a FixK1 mutant exhibit wild-type levels of hydrogenase activity. In beta-galactosidase transcriptional assays with NifA and FixK2 expression plasmids, the FixK2 protein induces transcription from the hup promoter to levels similar to those induced by HoxA, the transcriptional activator of free-living hydrogenase expression. The NifA protein does not activate transcription at the hydrogenase promoter. Therefore, FixK2 is involved in the transcriptional activation of symbiotic hydrogenase expression. By using beta-galactosidase transcriptional fusion constructs containing successive truncations of the hup promoter, the region of the hup promoter required for regulation by FixK2 was determined to be between 29 and 44 bp upstream of the transcription start site.

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    Stevenson University Library
    1525 Greenspring Valley Road
    Stevenson, MD 21153

    Website: http://stevensonlibrary.org

    Contact information:
    Elizabeth Fields
    efields2@stevenson.edu
    http://stevenson.libguides.com/sse


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.