The FixK2 protein is involved in regulation of symbiotic hydrogenase expression in Bradyrhizobium japonicum
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Metadata
Show full item recordDate
1998-06Type of Work
4 pagesText
journal articles
Department
Beverly K. Fine School of the SciencesCitation of Original Publication
Durmowicz, M. C., & Maier, R. J. (1998). The FixK2 protein is involved in regulation of symbiotic hydrogenase expression in Bradyrhizobium japonicum. Journal Of Bacteriology, 180(12), 3253-3256.Subjects
Genes, BacterialBacterial Proteins/*metabolism
Hydrogenase/*genetics
Rhizobiaceae/*genetics
Rhizobiaceae/*metabolism
Bacterial Proteins/genetics
Base Sequence
Binding Sites/genetics
DNA, Bacterial/genetics
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Molecular Sequence Data
Mutation
Nitrogen Fixation/genetics
Phenotype
Promoter Regions, Genetic
Symbiosis
Transcriptional Activation
Abstract
The roles of the nitrogen fixation regulatory proteins NifA, FixK1, and FixK2 in the symbiotic regulation of hydrogenase structural gene expression in Bradyrhizobium japonicum have been investigated. Bacteroids from FixJ and FixK2 mutants have little or no hydrogenase activity, and extracts from these mutant bacteroids contain no hydrogenase protein. Bacteroids from a FixK1 mutant exhibit wild-type levels of hydrogenase activity. In beta-galactosidase transcriptional assays with NifA and FixK2 expression plasmids, the FixK2 protein induces transcription from the hup promoter to levels similar to those induced by HoxA, the transcriptional activator of free-living hydrogenase expression. The NifA protein does not activate transcription at the hydrogenase promoter. Therefore, FixK2 is involved in the transcriptional activation of symbiotic hydrogenase expression. By using beta-galactosidase transcriptional fusion constructs containing successive truncations of the hup promoter, the region of the hup promoter required for regulation by FixK2 was determined to be between 29 and 44 bp upstream of the transcription start site.
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