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    Effect of Viscoelasticity on the Analysis of Single-Molecule Force Spectroscopy on Live Cells

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    1-s2.0-S0006349512006248-main.pdf (654.7Kb)
    SupportingMethod.pdf (457.5Kb)
    Links to Files
    https://www.sciencedirect.com/science/article/pii/S0006349512006248?via%3Dihub
    Permanent Link
    https://doi.org/10.1016/j.bpj.2012.05.044
    http://hdl.handle.net/11603/11627
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    • UMBC Faculty Collection
    • UMBC Mechanical Engineering Department
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    Author/Creator
    Gupta, V.K.
    Neeves, K.B.
    Eggleton, C.D.
    Date
    2012-07-03
    Type of Work
    9 pages
    Text
    journal article
    Citation of Original Publication
    V.K. Gupta , K.B. Neeves , C.D. Eggleton , Effect of Viscoelasticity on the Analysis of Single-Molecule Force Spectroscopy on Live Cells, Biophysical Journal Volume 103, Issue 1, 3 July 2012, Pages 137-145, https://doi.org/10.1016/j.bpj.2012.05.044
    Rights
    This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
    Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0)
    https://creativecommons.org/licenses/by-nc-nd/3.0/
    Subjects
    Viscoelasticity
    Single-Molecule Force
    Spectroscopy
    Live Cells
    receptor-ligand bonds
    UMBC High Performance Computing Facility (HPCF)
    Abstract
    Single-molecule force spectroscopy is used to probe the kinetics of receptor-ligand bonds by applying mechanical forces to an intermediate media on which the molecules reside. When this intermediate media is a live cell, the viscoelastic properties can affect the calculation of rate constants. We theoretically investigate the effect of media viscoelasticity on the common assumption that the bond force is equal to the instantaneous applied force. Dynamic force spectroscopy is simulated between two cells of varying micromechanical properties adhered by a single bond with a constant kinetic off-rate. We show that cell and microvilli deformation, and hydrodynamic drag contribute to bond forces that can be 28–90% lower than the applied force for loading rates of 10³–10⁷ pN/s, resulting in longer bond lifetimes. These longer bond lifetimes are not caused by changes in bond kinetics; rather, they are due to the mechanical response of the intermediate media on which the bonds reside. Under the assumption that the instantaneous bond force is equal to the applied force—thereby ignoring viscoelasticity—leads to 14–39% error in the determination of off-rates. We present an approach that incorporates viscoelastic properties in calculating the instantaneous bond force and kinetic dissociation parameter of the intermolecular bond.


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    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3544


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.