Fluorescent silica particles for monitoring oxygen levels in three‐dimensional heterogeneous cellular structures
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Miguel A. Acosta, Melissa Velasquez , Katelyn Williams, Julia M. Ross, Jennie B. Leach, Fluorescent silica particles for monitoring oxygen levels in three‐dimensional heterogeneous cellular structures, Biotechnology and Bioengineering, Volume 109, Issue 10, 2012, https://doi.org/10.1002/bit.24530
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This is the peer reviewed version of the following article: Miguel A. Acosta, Melissa Velasquez , Katelyn Williams, Julia M. Ross, Jennie B. Leach, Fluorescent silica particles for monitoring oxygen levels in three‐dimensional heterogeneous cellular structures, Biotechnology and Bioengineering, Volume 109, Issue 10, 2012, https://doi.org/10.1002/bit.24530, which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/bit.24530. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
This is the peer reviewed version of the following article: Miguel A. Acosta, Melissa Velasquez , Katelyn Williams, Julia M. Ross, Jennie B. Leach, Fluorescent silica particles for monitoring oxygen levels in three‐dimensional heterogeneous cellular structures, Biotechnology and Bioengineering, Volume 109, Issue 10, 2012, https://doi.org/10.1002/bit.24530, which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/bit.24530. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
Abstract
Bacterial biofilms are a major obstacle challenging the development of more effective therapies to
treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm
formation and planktonic cell detachment; however, there are insufficient tools available to
measure oxygen concentrations within complex three-dimensional structures with ~1 μm
resolution. Such measurements may complement measures of biofilm structure and cell activity to
provide a more comprehensive understanding of biofilm biology. Thus, we developed oxygensensing
microparticles specifically designed to characterize oxygen transport through the volume
of bacterial biofilms. The Stöber method was used to synthesize monodisperse silica
microparticles of approximately the same size as a bacterium (~1 μm). Two fluorophores, oxygensensitive
Ru(Ph₂phen₃)Cl₂, and the reference fluorophore Nile blue chloride were immobilized on
the surface of the particles. We demonstrate application of the microparticles toward measuring
the oxygen concentration profiles within a live Staphylococcus aureus biofilm.