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    Fluorescent silica particles for monitoring oxygen levels in three‐dimensional heterogeneous cellular structures

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    nihms372698.pdf (2.861Mb)
    Links to Files
    https://onlinelibrary.wiley.com/doi/full/10.1002/bit.24530
    Permanent Link
    https://doi.org/10.1002/bit.24530
    http://hdl.handle.net/11603/12283
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    • UMBC Chemical, Biochemical & Environmental Engineering Department
    • UMBC Faculty Collection
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    Author/Creator
    Acosta, Miguel A.
    Velasquez, Melissa
    Williams, Katelyn
    Ross, Julia M.
    Leach, Jennie B.
    Date
    2012-04-17
    Type of Work
    17 pages
    Text
    journal articles postprints
    Citation of Original Publication
    Miguel A. Acosta, Melissa Velasquez , Katelyn Williams, Julia M. Ross, Jennie B. Leach, Fluorescent silica particles for monitoring oxygen levels in three‐dimensional heterogeneous cellular structures, Biotechnology and Bioengineering, Volume 109, Issue 10, 2012, https://doi.org/10.1002/bit.24530
    Rights
    This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
    This is the peer reviewed version of the following article: Miguel A. Acosta, Melissa Velasquez , Katelyn Williams, Julia M. Ross, Jennie B. Leach, Fluorescent silica particles for monitoring oxygen levels in three‐dimensional heterogeneous cellular structures, Biotechnology and Bioengineering, Volume 109, Issue 10, 2012, https://doi.org/10.1002/bit.24530, which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/bit.24530. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
    Subjects
    Staphylococcus aureus
    biofilms
    Stöber synthesis
    microparticles
    oxygen sensors
    Abstract
    Bacterial biofilms are a major obstacle challenging the development of more effective therapies to treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm formation and planktonic cell detachment; however, there are insufficient tools available to measure oxygen concentrations within complex three-dimensional structures with ~1 μm resolution. Such measurements may complement measures of biofilm structure and cell activity to provide a more comprehensive understanding of biofilm biology. Thus, we developed oxygensensing microparticles specifically designed to characterize oxygen transport through the volume of bacterial biofilms. The Stöber method was used to synthesize monodisperse silica microparticles of approximately the same size as a bacterium (~1 μm). Two fluorophores, oxygensensitive Ru(Ph₂phen₃)Cl₂, and the reference fluorophore Nile blue chloride were immobilized on the surface of the particles. We demonstrate application of the microparticles toward measuring the oxygen concentration profiles within a live Staphylococcus aureus biofilm.


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    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3021


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.