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    Altered structural and mechanical properties in decellularized rabbit carotid arteries

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    nihms82906.pdf (2.896Mb)
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    https://www.sciencedirect.com/science/article/pii/S1742706108003784?via%3Dihub#!
    Permanent Link
    https://doi.org/10.1016/j.actbio.2008.11.028
    http://hdl.handle.net/11603/12289
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    • UMBC Chemical, Biochemical & Environmental Engineering Department
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    Author/Creator
    Williams, C.
    Liao, J.
    Joyce, E.M.
    Wang, B.
    Leach, J.B.
    Sacks, M.S.
    Wong, J.Y.
    Date
    2008-12-11
    Type of Work
    22 pages
    Text
    journal articles postprints
    Citation of Original Publication
    C. Williams , J. Liao, E.M. Joyce , B. Wang , J.B. Leach , M.S. Sacks , J.Y. Wong , Altered structural and mechanical properties in decellularized rabbit carotid arteries, Acta Biomaterialia Volume 5, Issue 4, 2008, https://doi.org/10.1016/j.actbio.2008.11.028
    Rights
    This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
    Subjects
    decellularized artery
    extracellular matrix
    small-angle light scattering
    fiber orientation
    mechanical properties
    Abstract
    Recently, major achievements in creating decellularized whole tissue scaffolds have drawn considerable attention to decellularization as a promising approach for tissue engineering. Decellularized tissues are expected to have mechanical strength and structure similar to the native tissues from which they are derived. However, numerous studies have shown that mechanical properties change after decellularization. Often, tissue structure is observed by histology and electron microscopy, but the structural alterations that may have occurred are not always evident. Here, a variety of techniques were used to investigate changes in tissue structure and relate them to altered mechanical behavior in decellularized rabbit carotid arteries. Histology and scanning electrom microscopy revealed that major extracellular matrix components were preserved and fibers appeared intact, although collagen appeared looser and less crimped after decellularization. Transmission electrom microscopy confirmed the presence of proteoglycans (PG), but there was decreased PG density and increased spacing between collagen fibrils. Mechanical testing and opening angle measurements showed that decellularized arteries had significantly increased stiffness, decreased extensibility and decreased residual stress compared with native arteries. Small-angle light scattering revealed that fibers had increased mobility and that structural integrity was compromised in decellularized arteries. Taken together, these studies revealed structural alterations that could be related to changes in mechanical properties. Further studies are warranted to determine the specific effects of different decellularization methods on the structure and performance of decellularized arteries used as vascular grafts.


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    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3544


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.