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dc.contributor.authorCitarasu, Thavasimuthu
dc.contributor.authorLelin, Chinnadurai
dc.contributor.authorThirumalaikumar, Eswaramoorthy
dc.contributor.authorBabu, Mariavincent Michael
dc.contributor.authorVakharia, Vikram N.
dc.date.accessioned2019-02-14T15:54:54Z
dc.date.available2019-02-14T15:54:54Z
dc.date.issued2019-03
dc.description.abstractMacrobrachium rosenbergii Nodavirus (MrNV) causes white tail disease (WTD) in Giant freshwater prawn Macrobrachium rosenbergii which leads to immense economic losses in hatcheries and farms. In the present study, we cloned the capsid protein gene of MrNV-CP-RNA-2 (1146 bp) into a DNA vaccine vector pVAX1 to form MrNV-CP-RNA-2- pVAX1. The bacterial transformant, containing the MrNV-CP gene, was coated on the fish diet pellets and fed to juvenile M. rosenbergii for 40 days. After the vaccine delivery, group of M. rosenbergii were challenged with virulent MrNV on 20 and 40th days post-vaccination (dpv) respectively and monitored for the survival. The non-vaccinated M. rosenbergii succumbed to death (100%) within 5 days, whereas the MrNV-CP-RNA-2- pVAX1 treated groups had the survivals of 60 and 80% in 20 and 40 dpv respectively (P ≤ 0.001). To study the MrNV infection level, double step PCR was performed at different dpv. The results revealed that in 20 dpv group, the infection was decreased to 65% and in 40 dpv group the infection decreased to 69% from control diet fed prawns (P < 0.001). Haematological parameters like coagulation time, total haemocyte count (THC) and oxyhaemocyanin levels were performed for the control and vaccinated prawns. The vaccination helped to decrease the time of coagulation, improved THC and oxyhaemocyanin levels at a significant level (p < 0.001) when compared to the non-vaccinated group. The immunological parameters like prophenol oxidase (ProPO), superoxide anion and intra-agar lysozyme activity were also performed and the results revealed that the level of proPO, superoxide anion and lysozyme activities were significantly (P ≤ 0.05) increased in 20 and 40 dpv groups respectively, when compared with the non-vaccinated groups. Based on the vaccination trials, the DNA vaccine construct MrNV-CP-RNA-2-pVAX1 effectively improved the survival against MrNV challenge, helped to decrease viral load and enhanced the immune system to protect the prawn from MrNV infection. This vaccine construct is highly useful to protect the M. rosenbergii from MrNV infection.en_US
dc.description.sponsorshipDr. T. Citarasu gratefully acknowledges University Grants Commission (UGC), Government of India, New Delhi for the fi nancial support, in the form of Raman Postdoctoral Fellowship (No.F. 5-37/ 2014 (IC) dtd 26-08-2014) and Institute of Marine and Environmental Technology (IMET), University of Maryland, Baltimore County, Baltimore, Maryland (MD), United States of America for the host lab support.en_US
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S105046481830768Xen_US
dc.format.extent8 pagesen_US
dc.genrejournal articles postprintsen_US
dc.identifierdoi:10.13016/m27d94-a8hb
dc.identifier.citationThavasimuthu Citarasu, Chinnadurai Lelin , Eswaramoorthy Thirumalaikumar, Mariavincent Michael Babu , Vikram N. Vakharia, Macrobrachium rosenbergii nodavirus (MrNV)-CP-RNA-2 DNA vaccine confers protective immunity in giant freshwater prawn Macrobrachium rosenbergii against MrNV infection, Fish & Shellfish Immunology Volume 86, March 2019, Pages 319-326, https://doi.org/10.1016/j.fsi.2018.11.049en_US
dc.identifier.urihttps://doi.org/10.1016/j.fsi.2018.11.049
dc.identifier.urihttp://hdl.handle.net/11603/12787
dc.language.isoen_USen_US
dc.publisherElsevier B.V.en_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/us/
dc.rights.uriAccess to this item will begin on on March 31. 2020*
dc.subjectDNA vaccineen_US
dc.subjectgiant freshwater prawnen_US
dc.subjectmacrobrachium rosenbergii nodavirus ( Mr NV)en_US
dc.subjectprotective immunityen_US
dc.titleMacrobrachium rosenbergii nodavirus ( Mr NV)-CP-RNA-2 DNA vaccine confers protective immunity in giant freshwater prawn Macrobrachium rosenbergii against Mr NV infectionen_US
dc.typeTexten_US


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