Interaction of the Bacillus subtilis glnRA repressor with operator and promoter sequences in vivo
Links to Fileshttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC206142/
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Type of Work11 pages
Citation of Original PublicationJ. C. Gutowski and H. J. Schreier, Interaction of the Bacillus subtilis glnRA repressor with operator and promoter sequences in vivo, J Bacteriol. 1992 Feb; 174(3): 671–681, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC206142/
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SubjectsglnRA expression under nitrogen-limiting (derepressing)
Bacillus subtilis glnRA
vivo dimethyl sulfate footprinting
In vivo dimethyl sulfate footprinting of the Bacillus subtilis ginRA regulatory region under repressing and derepressing conditions demonstrated that the GlnR protein, encoded by glnR, interacts with two sites situated within and adjacent to the gInRA promoter. One site, glnRAol, between positions -40 and -60 relative to the start point of transcription, is a 21-bp symmetrical element that has been identified as essential for glnRA regulation (H. J. Schreier, C. A. Rostkowski, J. F. Nomellini, and K. D. Hirschi, J. Mol. Biol. 220:241-253, 1991). The second site, glnRAo2, is a quasisymmetrical element having partial homology to glnRAo, and is located within the promoter between positions -17 and -37. The symmetry and extent of modifications observed for each site during repression and derepression indicated that GlnR interacts with the glnRA regulatory region by binding to both sites in approximately the same manner. Experiments using potassium permanganate to probe open complex formation by RNA polymerase demonstrated that transcriptional initiation is inhibited by GlnR. Furthermore, distortion of the DNA helix within glnRAo2 occurred upon GlnR binding. While glutamine synthetase, encoded by glnA, has been implicated in controlling ginRA expression, analyses with dimethyl sulfate and potassium permanganate ruled out a role for glutamine synthetase in directly influencing transcription by binding to operator and promoter regions. Our results suggested that inhibition of transcription from the glnRA promoter involves GlnR occupancy at both glnRAo₁ and glnRAo₂. In addition, modification of bases within the glnRAo₂ operator indicated that control ofginRA expression under nitrogen-limiting (derepressing) conditions included the involvement of a factor(s) other than GlnR.