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dc.contributor.authorSchreier, Harold J.
dc.contributor.authorSmith, Thomas M.
dc.contributor.authorBernlohr, Robert W.
dc.date.accessioned2019-03-22T14:38:08Z
dc.date.available2019-03-22T14:38:08Z
dc.date.issued1982-08
dc.description.abstractThe levels of the inducible nitrogen catabolic enzymes arginase (L-arginine amidinohydrolase, EC 3.5.3.1) and alanine dehydrogenase (L-alanine:NAD+ oxidoreductase [deaminating], EC 1.4.1.1) from Bacillus licheniformis and histidase (L-histidine ammonia-lyase, EC 4.3.1.3) from Bacillus subtilis and the ammonia assimilatory enzymes from B. licheniformis were determined in cultures grown in the presence of different nitrogen sources. Although the levels of these enzymes were dependent upon the nitrogen source present, induction of the catabolic enzymes in response to the addition of inducer occurred even in the presence of preferred nitrogen sources. Intracellular pool sizes of ammonia, glutamate, glutamine, and alpha-ketoglutarate were measured in continuous cultures of b. licheniformis growing in the presence of different nitrogen sources. A comparison of the pool sizes of these metabolites with the ammonia assimilatory enzyme levels showed that the pools of the metabolites did not change in a manner consistent with their use as regulators of the synthesis of any of these enzymes.en_US
dc.description.sponsorshipThis work was supported in part by grants PCM78-23938 and PCM79-24644 from the National Science Foundation and 2405 from the Pennsylvania Agricultural Experiment Station. We thank J. E. Brenchley, T. J. Donohue, and M. S. Ruppen, who were involved in early stages of this work, and A. T. Phillips and S. E. Stevens, Jr., for helpful discussions.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/6124533en_US
dc.format.extent5 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2zmx7-59jp
dc.identifier.citationH J Schreier, T M Smith, and R W Bernlohr, Regulation of nitrogen catabolic enzymes in Bacillus spp, J Bacteriol. 1982 Aug; 151(2): 971–975, https://www.ncbi.nlm.nih.gov/pubmed/6124533en_US
dc.identifier.urihttp://hdl.handle.net/11603/13124
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Biological Sciences Department
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.titleRegulation of nitrogen catabolic enzymes in Bacillus sppen_US
dc.typeTexten_US


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