Specialized transducing phages for ribosomal protein genes of Escherichia coli

Thumbnail Image

Links to Files

https://www.pnas.org/content/72/1/6

Permanent Link

https://doi.org/10.1073/pnas.72.1.6
 http://hdl.handle.net/11603/14323

Collections

UMBC Biological Sciences Department
UMBC Faculty Collection

Author/Creator

Author/Creator ORCID

Date

1975-01-01

Type of Work

journal articles
Text

Department

Program

Citation of Original Publication

S R Jaskunas, L Lindahl, and M Nomura, Specialized transducing phages for ribosomal protein genes of Escherichia coli, PNAS January 1, 1975 72 (1) 6-10; https://doi.org/10.1073/pnas.72.1.6

Rights

This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
Non-commercial use only
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Subjects

ribosomal protein genes
Escherichia coli
bacterial chromosome
lambdadspc2

Abstract

Specialized lambda transducing phages have been isolated carrying approximately half the ribosomal protein genes of E. coli. These phages carry regions of the bacterial chromosome between aroE and fus. The ribosomal protein genes on these phages have been identified by the stimulation of ribosomal protein synthesis in ultraviolet-irradiated bacteria following infection by the transducing phage, and by the in vitro synthesis of ribosomal proteins in a DNA-dependent protein synthesizing system. The results indicate lambdadspcl probably carries at least 22 ribosomal protein genes and lambdadspc2 at least 26 genes. All these genes are clustered between trkA and strA. At least 13 of them have not been previously mapped.