Screen Printing to Create 3D Tissue Models
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Date
2020-11-02
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Narendra Pandala, Sydney Haywood, Michael A. LaScola, Adam Day, Joshua Leckron, and Erin Lavik ACS Applied Bio Materials 2020 3 (11), 8113-8120 DOI: 10.1021/acsabm.0c01256
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This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in ACS Applied Bio Materials, copyright © American Chemical Society after peer review. To access the final edited and published work see https://doi.org/10.1021/acsabm.0c01256.
This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in ACS Applied Bio Materials, copyright © American Chemical Society after peer review. To access the final edited and published work see https://doi.org/10.1021/acsabm.0c01256.
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Abstract
3D printing has revolutionized making tissue models, but the instruments are often quite expensive, and the approach can involve heat and/or shear forces that can damage cells. As a complement to more traditional 3D printing approaches, we looked at screen printing. Screen printing is an additive manufacturing technique used to pattern inks through screens supporting patterns onto different surfaces. It has a wide range of applications ranging from traditional printing to printing electric circuit boards. Taking cues from this we have developed a process of screen printing live cells along with a suitable scaffold on to different surfaces to generate in vitro models. The process is not only inexpensive and simple to use, but it also offers a wide range of advantages like the ability to use a range of bioinks limited only by their gelation time, printing on different surfaces, and the ability to autoclave all of the major components. In this paper, we present the screen assembly and the setup we used to print the cells along with the resolution and limits of features printed and the effect of the printing on the cells.