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    Maintenance of Rat Taste Buds in Primary Culture

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    https://academic.oup.com/chemse/article/26/7/861/326886
    Permanent Link
    https://doi.org/10.1093/chemse/26.7.861
    http://hdl.handle.net/11603/21074
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    • UMBC Biological Sciences Department
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    Author/Creator
    Ruiz, Collin J.
    Stone, Leslie M.
    McPheeters, Martha
    Ogura, Tatsuya
    Böttger, Bärbel
    Lasher, Robert S.
    Finger, Thomas E.
    Kinnamon, Sue C.
    Date
    2001-09-01
    Type of Work
    13 pages
    Text
    journal articles
    Citation of Original Publication
    Collin J. Ruiz, Leslie M. Stone, Martha McPheeters, Tatsuya Ogura, Bärbel Böttger, Robert S. Lasher, Thomas E. Finger, Sue C. Kinnamon, Maintenance of Rat Taste Buds in Primary Culture, Chemical Senses, Volume 26, Issue 7, September 2001, Pages 861–873, https://doi.org/10.1093/chemse/26.7.861
    Rights
    This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
    Subjects
    taste buds
    isolated rat taste buds
    taste cells
    animals
    culture medium
    Abstract
    The differentiated taste bud is a complex end organ consisting of multiple cell types with various morphological, immunocytochemical and electrophysiological characteristics. Individual taste cells have a limited lifespan and are regularly replaced by a proliferative basal cell population. The specific factors contributing to the maintenance of a differentiated taste bud are largely unknown. Supporting isolated taste buds in culture would allow controlled investigation of factors relevant to taste bud survival. Here we describe the culture and maintenance of isolated rat taste buds at room temperature and at 37°C. Differentiated taste buds can be sustained for up to 14 days at room temperature and for 3–4 days at 37°C. Over these periods individual cells within the cultured buds maintain an elongated morphology. Further, the taste cells remain electrically excitable and retain various proteins indicative of a differentiated phenotype. Despite the apparent health of differentiated taste cells, cell division occurs for only a short period following plating, suggesting that proliferating cells in the taste bud are quickly affected by isolation and culture.


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    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3021


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.