Luminescent quantum dots for cellular analysis

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https://www.spiedigitallibrary.org/conference-proceedings-of-spie/6370/1/Luminescent-quantum-dots-for-cellular-analysis/10.1117/12.690646.short?SSO=1

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https://doi.org/10.1117/12.690646
 http://hdl.handle.net/11603/21350

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UMBC Chemistry & Biochemistry Department

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2006-10-19

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conference papers and proceedings
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Rosenzweig, Zeev; Shi, Lifang; Rosenzweig, Nitsa; Luminescent quantum dots for cellular analysis; Proceedings Volume 6370, Nanomaterial Synthesis and Integration for Sensors, Electronics, Photonics, and Electro-Optics; 637003 (2006); https://www.spiedigitallibrary.org/conference-proceedings-of-spie/6370/1/Luminescent-quantum-dots-for-cellular-analysis/10.1117/12.690646.short?SSO=1

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©2006 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited

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Abstract

The paper describes the fabrication, characterization and applications of novel luminescent quantum dots fluorescence resonance energy transfer (FRET) based enzymatic activity probes. The luminescent probes are based on FRET between luminescent quantum dots that serve as donors and rhodamine acceptors that are immobilized to the surface of the quantum dots through peptide linkers that contain selective enzymatic cleavage sites. Upon enzymatic cleavage of the peptide linkers the rhodamine molecules no longer provide an efficient energy transfer channel to the quantum dots, which lightes up the initially quenched the quantum dots. The quantum dots based probes were applied for detecting enzyme activity and screening enzyme inhibitors. They were also used for the measurement of extracellular matrix metallproteinases (MMPs) activity in normal and cancerous breast cells tissues.