FRET-based luminescence sensors for carbohydrates and glycoproteins analysis

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https://www.spiedigitallibrary.org/conference-proceedings-of-spie/5588/0000/FRET-based-luminescence-sensors-for-carbohydrates-and-glycoproteins-analysis/10.1117/12.570450.short

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https://doi.org/10.1117/12.570450
 http://hdl.handle.net/11603/21352

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UMBC Chemistry & Biochemistry Department

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2004-12-07

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conference papers and proceedings
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Rosenzweig, Zeev; Rosenzweig, Nitsa; Blagoi, Gabriela; FRET-based luminescence sensors for carbohydrates and glycoproteins analysis; Proceedings Volume 5588, Smart Medical and Biomedical Sensor Technology II; (2004); https://www.spiedigitallibrary.org/conference-proceedings-of-spie/5588/0000/FRET-based-luminescence-sensors-for-carbohydrates-and-glycoproteins-analysis/10.1117/12.570450.short

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©2004 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited

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Abstract

This paper describes the development of novel particle-based fluorescence resonance energy transfer (FRET) biosensors. It describes the fundamentals of FRET in heterogeneous systems and the application of the new sensors in monitoring the binding affinity of carbohydrates and glycoproteins to lectins, which are carbohydrate binding proteins. The sensing approach is based on FRET between fluorescein (donor) labeled lectin molecules, adsorbed on the surface of micrometric polymeric beads, and polymeric dextran molecules labeled with Texas Red (acceptor). The FRET signal of the sensor decreases in the presence of carbohydrates or glycoproteins that inhibit the binding of Texas Red-labeled dextran molecules to the lectinic binding sites. The new FRET sensors could discriminate between carbohydrates and glycoproteins based on their binding affinity to the FRET sensing particles. Thery were also used for quantitative analysis of carbohydrates and glycoproteins in aqueous samples.