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    Set5 and Set1 cooperate to repress gene expression at telomeres and retrotransposons

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    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121362/
    Permanent Link
    https://doi.org/10.4161%2Fepi.27645
    http://hdl.handle.net/11603/26702
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    • UMBC Biological Sciences Department
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    Author/Creator
    Martín, Glòria Mas
    King, Devin A
    Green, Erin
    Garcia-Nieto, Pablo E
    Alexander, Richard
    Collins, Sean R
    Krogan, Nevan J
    Gozani, Or P
    Morrison, Ashby J
    Author/Creator ORCID
    https://orcid.org/0000-0003-3923-6726
    Date
    2014-01-17
    Type of Work
    10 pages
    Text
    journal articles
    Citation of Original Publication
    Martín, Glòria Mas et al. “Set5 and Set1 cooperate to repress gene expression at telomeres and retrotransposons.” Epigenetics vol. 9,4 (2014): 513-22. doi:10.4161/epi.27645
    Rights
    This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
    Subjects
    transcription
    genetic interaction
    Set5
    Set1
    histone methylation
    telomeres
    retrotransposons
    Abstract
    A complex interplay between multiple chromatin modifiers is critical for cells to regulate chromatin structure and accessibility during essential DNA-templated processes such as transcription. However, the coordinated activities of these chromatin modifiers in the regulation of gene expression are not fully understood. We previously determined that the budding yeast histone H4 methyltransferase Set5 functions together with Set1, the H3K4 methyltransferase, in specific cellular contexts. Here, we sought to understand the relationship between these evolutionarily conserved enzymes in the regulation of gene expression. We generated a comprehensive genetic interaction map of the functionally uncharacterized Set5 methyltransferase and expanded the existing genetic interactome of the global chromatin modifier Set1, revealing functional overlap of the two enzymes in chromatin-related networks, such as transcription. Furthermore, gene expression profiling via RNA-Seq revealed an unexpected synergistic role of Set1 and Set5 in repressing transcription of Ty transposable elements and genes located in subtelomeric regions. This study uncovers novel pathways in which the methyltransferase Set5 participates and, more importantly, reveals a partnership between Set1 and Set5 in transcriptional repression near repetitive DNA elements in budding yeast. Together, our results define a new functional relationship between histone H3 and H4 methyltransferases, whose combined activity may be implicated in preserving genomic integrity.


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    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3021


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.