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    Overlapping Regulation of CenH3 Localization and Histone H3 Turnover by CAF-1 and HIR Proteins in Saccharomyces cerevisiae

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    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018296/
    Permanent Link
    https://doi.org/10.1534%2Fgenetics.110.123117
    http://hdl.handle.net/11603/26704
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    • UMBC Biological Sciences Department
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    Author/Creator
    da Rosa, Jessica Lopes
    Holik, John
    Green, Erin
    Rando, Oliver J.
    Kaufman, Paul D.
    Author/Creator ORCID
    https://orcid.org/0000-0003-3923-6726
    Date
    2011-01
    Type of Work
    13 pages
    Text
    journal articles
    Citation of Original Publication
    Lopes da Rosa, Jessica et al. “Overlapping regulation of CenH3 localization and histone H3 turnover by CAF-1 and HIR proteins in Saccharomyces cerevisiae.” Genetics vol. 187,1 (2011): 9-19. doi:10.1534/genetics.110.123117
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    This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
    Subjects
    histone
    Cse4
    chromatin
    transcription
    Abstract
    Accurate chromosome segregation is dependent on the centromere-specific histone H3 isoform known generally as CenH3, or as Cse4 in budding yeast. Cytological experiments have shown that Cse4 appears at extracentromeric loci in yeast cells deficient for both the CAF-1 and HIR histone H3/H4 deposition complexes, consistent with increased nondisjunction in these double mutant cells. Here, we examined molecular aspects of this Cse4 mislocalization. Genome-scale chromatin immunoprecipitation analyses demonstrated broader distribution of Cse4 outside of centromeres in cac1Δ hir1Δ double mutant cells that lack both CAF-1 and HIR complexes than in either single mutant. However, cytological localization showed that the essential inner kinetochore component Mif2 (CENP-C) was not recruited to extracentromeric Cse4 in cac1Δ hir1Δ double mutant cells. We also observed that rpb1-1 mutants displayed a modestly increased Cse4 half-life at nonpermissive temperatures, suggesting that turnover of Cse4 is partially dependent on Pol II transcription. We used genome-scale assays to demonstrate that the CAF-1 and HIR complexes independently stimulate replication-independent histone H3 turnover rates. We discuss ways in which altered histone exchange kinetics may affect eviction of Cse4 from noncentromeric loci.


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    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3021


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.