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    The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region

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    56.pdf (1.073Mb)
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    http://hdl.handle.net/11603/275
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    • UMBC Chemical, Biochemical & Environmental Engineering Department
    • UMBC Faculty Collection
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    Author/Creator
    Bush, C Allen
    Chen, Yuansha
    Bystricky, Peter
    Adeyeye, Jacob
    Panigrahi, Pinaki
    Ali, Afsar
    Johnson, Judith A
    Morris, JG
    Stine, OC
    Date
    2007-03-15
    2013-01-02
    2007
    Type of Work
    application/pdf
    File Size: 1125911 Bytes
    Text
    research articles
    Department
    Chemical, Biochemical & Environmental Engineering
    Citation of Original Publication
    Bush, C. A., et al. "The Capsule Polysaccharide Structure and Biogenesis for Non-O1 Vibrio Cholerae NRT36S: Genes Are Embedded in the LPS Region." BMC Microbiology., 15 Mar. 2007. 02 Jan. 2013 <
    Rights
    This item may be protected under Title 17 of the U.S. Copyright Law. It is made available by UMBC for non-commercial research and education. For permission to publish or reproduce, please contact the author.
    Subjects
    capsular (CPS) polysaccharide
    LPS biosynthesis genes
    V. cholerae
    surface polysaccharide
    biogenesis
    Abstract
    Background: In V. cholerae, the biogenesis of capsule polysaccharide is poorly understood. The elucidation of capsule structure and biogenesis is critical to understanding the evolution of surface polysaccharide and the internal relationship between the capsule and LPS in this species. V. cholerae serogroup O31 NRT36S, a human pathogen that produces a heat-stable enterotoxin (NAG-ST), is encapsulated. Here, we report the covalent structure and studies of the biogenesis of the capsule in V. cholerae NRT36S. Results: The structure of the capsular (CPS) polysaccharide was determined by high resolution NMR spectroscopy and shown to be a complex structure with four residues in the repeating subunit. The gene cluster of capsule biogenesis was identified by transposon mutagenesis combined with whole genome sequencing data (GenBank accession DQ915177). The capsule gene cluster shared the same genetic locus as that of the O-antigen of lipopolysaccharide (LPS) biogenesis gene cluster. Other than V. cholerae O139, this is the first V. cholerae CPS for which a structure has been fully elucidated and the genetic locus responsible for biosynthesis identified. Conclusion: The co-location of CPS and LPS biosynthesis genes was unexpected, and would provide a mechanism for simultaneous emergence of new O and K antigens in a single strain. This, in turn, may be a key element for V. cholerae to evolve new strains that can escape immunologic detection by host populations.


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    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3021


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.