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dcterms.accrualPolicyMade available via a Transforming Collections Grant funded by ALCTS, Association for Library Collections and Technical Services, to rescue threatened or endangered faculty publications.
dc.contributor.departmentChemical, Biochemical & Environmental Engineering
dc.creatorBush, C Allen
dc.creatorChen, Yuansha
dc.creatorBystricky, Peter
dc.creatorAdeyeye, Jacob
dc.creatorPanigrahi, Pinaki
dc.creatorAli, Afsar
dc.creatorJohnson, Judith A
dc.creatorMorris, JG
dc.creatorStine, OC
dc.date.accessioned2015-10-12T18:49:23Z
dc.date.available2015-10-12T18:49:23Z
dc.date.issued2007-03-15
dcterms.dateAccepted2013-01-02
dcterms.dateCopyrighted2007
dc.description.abstractBackground: In V. cholerae, the biogenesis of capsule polysaccharide is poorly understood. The elucidation of capsule structure and biogenesis is critical to understanding the evolution of surface polysaccharide and the internal relationship between the capsule and LPS in this species. V. cholerae serogroup O31 NRT36S, a human pathogen that produces a heat-stable enterotoxin (NAG-ST), is encapsulated. Here, we report the covalent structure and studies of the biogenesis of the capsule in V. cholerae NRT36S. Results: The structure of the capsular (CPS) polysaccharide was determined by high resolution NMR spectroscopy and shown to be a complex structure with four residues in the repeating subunit. The gene cluster of capsule biogenesis was identified by transposon mutagenesis combined with whole genome sequencing data (GenBank accession DQ915177). The capsule gene cluster shared the same genetic locus as that of the O-antigen of lipopolysaccharide (LPS) biogenesis gene cluster. Other than V. cholerae O139, this is the first V. cholerae CPS for which a structure has been fully elucidated and the genetic locus responsible for biosynthesis identified. Conclusion: The co-location of CPS and LPS biosynthesis genes was unexpected, and would provide a mechanism for simultaneous emergence of new O and K antigens in a single strain. This, in turn, may be a key element for V. cholerae to evolve new strains that can escape immunologic detection by host populations.
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dc.genreresearch articles
dc.identifierdoi:10.13016/M2BP92
dc.identifier.citationBush, C. A., et al. "The Capsule Polysaccharide Structure and Biogenesis for Non-O1 Vibrio Cholerae NRT36S: Genes Are Embedded in the LPS Region." BMC Microbiology., 15 Mar. 2007. 02 Jan. 2013 <
dc.identifier.urihttp://hdl.handle.net/11603/275
dc.languageen
dc.publisherOriginally published by: BMC Microbiology
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item may be protected under Title 17 of the U.S. Copyright Law. It is made available by UMBC for non-commercial research and education. For permission to publish or reproduce, please contact the author.
dc.sourceOriginal File Name: BushC01.pdf
dc.subjectcapsular (CPS) polysaccharide
dc.subjectLPS biosynthesis genes
dc.subjectV. cholerae
dc.subjectsurface polysaccharide
dc.subjectbiogenesis
dc.titleThe capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region
dc.typeText


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