A comparative evaluation of layer-by-layer assembly techniques for surface modification of microcarriers used in human mesenchymal stromal cell manufacturing
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Timsina, Hemanta, Jasmine McTyer, Raj R. Rao, and Jorge Almodovar. “A Comparative Evaluation of Layer-by-Layer Assembly Techniques for Surface Modification of Microcarriers Used in Human Mesenchymal Stromal Cell Manufacturing.” Biotechnology Journal 17, no. 8 (2022): 2100605. https://doi.org/10.1002/biot.202100605.
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This is the peer reviewed version of the following article: Timsina, Hemanta, Jasmine McTyer, Raj R. Rao, and Jorge Almodovar. “A Comparative Evaluation of Layer-by-Layer Assembly Techniques for Surface Modification of Microcarriers Used in Human Mesenchymal Stromal Cell Manufacturing.” Biotechnology Journal 17, no. 8 (2022): 2100605. https://doi.org/10.1002/biot.202100605., which has been published in final form at https://doi.org/10.1002/biot.202100605. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Abstract
The demand for large quantities of highly potent human mesenchymal stromal cells (hMSCs) is growing given their therapeutic potential. To meet high production needs, suspension-based cell cultures using microcarriers are commonly used. Microcarriers are commonly made of or coated with extracellular matrix proteins or charged compounds to promote cell adhesion and proliferation. In this work, a simple method (draining filter) to perform layer by layer (LbL) assembly on microcarriers to create multilayers of heparin and collagen and further demonstrate that these multilayers have a positive effect on hMSC viability after 48 h of culture was demonstrated. The draining filter method is evaluated against two other methods found in literature—centrifugation and fluidized bed, showing that the draining filter method can perform the surface modification with greater efficiency and with less materials and steps needed in the coating process.