Intracellular Measurements in Mammary Carcinoma Cells Using Fiber-Optic Nanosensors

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dc.contributor.authorCullum, Brian
dc.contributor.authorGriffin, Guy D.
dc.contributor.authorMiller, Gordon H.
dc.contributor.authorVo-Dinh, Tuan
dc.date.accessioned2024-09-04T19:59:23Z
dc.date.available2024-09-04T19:59:23Z
dc.date.issued2000-01-01
dc.description.abstractSubmicrometer fiber-optic biosensors have been developed and used to measure toxic chemicals within single cells. Optical fibers that have been pulled to a distal-end diameter of less than 1 μm are coated with antibodies to selectively bind the species of interest. This paper describes the use of these fibers to selectively measure the concentration of benzo[a]pyrene tetrol (BPT), a metabolite of benzo[a]pyrene, within individual cells of two different cell lines, human mammary carcinoma cells and rat liver epithelial cells. The results from these measurements have been used to determine the sensitivity, reproducibility, and usefulness of these nanosensors. The detection limit of these biosensors has been determined to be 0.64 ± 0.17 × 10⁻¹¹ M for BPT.
dc.description.sponsorshipThis research is sponsored in part by the LDRD project (advanced nanosensors) as well as the Office of Biological and Environmental Research, U.S. Department of Energy under Contract DE-AC05-960R22464 with Lockheed Martin Energy Research Corp. In addition, B. Cullum is also supported by an appointment to the Oak Ridge National Laboratory Postdoctoral Research Associates Program administered jointly by the Oak Ridge National Laboratory and the Oak Ridge Institute for Science and Education.
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S0003269799943415
dc.format.extent8 pages
dc.genrejournal articles
dc.identifierdoi:10.13016/m25tv4-ehyz
dc.identifier.citationCullum, Brian M., Guy D. Griffin, Gordon H. Miller, and Tuan Vo-Dinh. “Intracellular Measurements in Mammary Carcinoma Cells Using Fiber-Optic Nanosensors.” Analytical Biochemistry 277, no. 1 (January 1, 2000): 25–32. https://doi.org/10.1006/abio.1999.4341.
dc.identifier.urihttps://doi.org/10.1006/abio.1999.4341
dc.identifier.urihttp://hdl.handle.net/11603/36055
dc.language.isoen_US
dc.publisherElsevier
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemistry & Biochemistry Department
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.
dc.rightsPublic Domain
dc.rights.urihttps://creativecommons.org/publicdomain/mark/1.0/
dc.subjectcarcinogen
dc.subjectbenzo[]pyrene
dc.subjectbenzo[]pyrene tetrol
dc.subjectbiosensor
dc.subjectintracellular measurements
dc.subjectnanosensor
dc.subjectfiber-optic
dc.subjectcancer
dc.titleIntracellular Measurements in Mammary Carcinoma Cells Using Fiber-Optic Nanosensors
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0002-5250-8290

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