Set4 regulates stress response genes and coordinates histone deacetylases within yeast subtelomeres

dc.contributor.authorJethmalani, Yogita
dc.contributor.authorTran, Khoa
dc.contributor.authorNegesse, Maraki
dc.contributor.authorSun, Winny
dc.contributor.authorRamos, Mark
dc.contributor.authorJaiswal, Deepika
dc.contributor.authorJezek, Meagan
dc.contributor.authorAmos, Shandon
dc.contributor.authorGarcia, Eric Joshua
dc.contributor.authorPark, DoHwan
dc.contributor.authorGreen, Erin
dc.date.accessioned2023-01-25T15:11:46Z
dc.date.available2023-01-25T15:11:46Z
dc.date.issued2021-10-08
dc.description.abstractThe yeast chromatin protein Set4 is a member of the Set3-subfamily of SET domain proteins which play critical roles in the regulation of gene expression in diverse developmental and environmental contexts. We previously reported that Set4 promotes survival during oxidative stress and regulates expression of stress response genes via stress-dependent chromatin localization. In this study, global gene expression analysis and investigation of histone modification status identified a role for Set4 in maintaining gene repressive mechanisms within yeast subtelomeres under both normal and stress conditions. We show that Set4 works in a partially overlapping pathway to the SIR complex and the histone deacetylase Rpd3 to maintain proper levels of histone acetylation and expression of stress response genes encoded in subtelomeres. This role for Set4 is particularly critical for cells under hypoxic conditions, where the loss of Set4 decreases cell fitness and cell wall integrity. These findings uncover a new regulator of subtelomeric chromatin that is key to stress defense pathways and demonstrate a function for Set4 in regulating repressive, heterochromatin-like environments.en_US
dc.description.sponsorshipThe authors thank all members of the Green lab for helpful discussions, technical assistance, and feedback on the manuscript. We thank Dr. Paul Kaufman for yeast strains and Dr. Philip Farabaugh for sharing equipment. We are also grateful to Tagide deCarvalho of the Keith R. Porter Imaging Facility for assistance with microscopy. This work was support by the National Institutes of Health (R01GM124342 to EM Green) and the National Research Foundation of Korea (NRF-2019H1D3A2A02102167 to D Park).en_US
dc.description.urihttps://www.life-science-alliance.org/content/4/12/e202101126en_US
dc.format.extent18 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2nppt-iz9q
dc.identifier.citationJethmalani, Yogita et al. “Set4 regulates stress response genes and coordinates histone deacetylases within yeast subtelomeres.” Life Science Alliance 4, No. 12 (8 October 2021). DOI: 10.26508/lsa.202101126.en_US
dc.identifier.urihttps://doi.org/10.26508/lsa.202101126
dc.identifier.urihttp://hdl.handle.net/11603/26707
dc.language.isoen_USen_US
dc.publisherLife Science Allianceen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.relation.ispartofUMBC Chemistry & Biochemistry Department
dc.relation.ispartofUMBC Mathematics and Statistics Department
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleSet4 regulates stress response genes and coordinates histone deacetylases within yeast subtelomeresen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0003-3422-8356en_US
dcterms.creatorhttps://orcid.org/0000-0002-2818-285Xen_US
dcterms.creatorhttps://orcid.org/0000-0002-2247-9951en_US
dcterms.creatorhttps://orcid.org/0000-0002-5069-2397en_US
dcterms.creatorhttps://orcid.org/0000-0002-6396-3563en_US
dcterms.creatorhttps://orcid.org/0000-0002-9132-5040en_US
dcterms.creatorhttps://orcid.org/0000-0003-3923-6726en_US

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