Induction of Reproductive Sterility in Coho Salmon (Oncorhynchus kisutch) by an Immersion-Based Gene Silencing Technology

dc.contributor.authorXu, Lan
dc.contributor.authorZhao, Mingli
dc.contributor.authorZohar, Yonathan
dc.contributor.authorWong, Ten-Tsao
dc.date.accessioned2023-12-01T17:56:07Z
dc.date.available2023-12-01T17:56:07Z
dc.date.issued2023-11-21
dc.description.abstractUndesired maturation and reproduction are major challenges in fish aquaculture regarding genetic introgression, precocious maturation, and reproduction-related mortality, which can have profound ecological or economic impacts. Farming reproductively sterile fish can effectively mitigate these challenges. In this paper, we transferred and applied a novel immersion-based, non-transgenic gene silencing technology to sterilize coho salmon for the first time. Unfertilized eggs were bath immersion-treated with csdnd-MO-Vivo in different immersion media. Eyed rates of treated groups ranged from 0.9 to 63.5%. Sterile fish lacking germ cells, and those with arrested germ cells/atretic oocytes, were obtained at 14 and 20 months of age, albeit at a low percentage (2.3 to 10.0% based on females). Gonadal histology and vasa/nanos3 gene expression profile were provided for comparing fertile and sterile gonads, as well as retarded ovaries. Future directions and strategies for optimizing the technology and improving sterility induction were also proposed. The successful production of sterile coho salmon achieved in this study demonstrates the proof of principle for this new sterilization technology. As we continue to expand upon these findings and refine the technology, achieving coho salmon sterile population farming would facilitate the future transfer and application to other commercially important aquaculture fish.
dc.description.sponsorshipThis research was funded by University of Maryland Baltimore County STRT7TEN-1113 to T.-T.W. In addition, L.X. and M.Z. were supported by scholarships from the China Scholarship Council.
dc.description.urihttps://mdpi.com/2077-1312/11/12/2208
dc.format.extent14 pages
dc.genrejournal articles
dc.identifier.citationXu, Lan, Mingli Zhao, Yonathan Zohar, and Ten-Tsao Wong. “Induction of Reproductive Sterility in Coho Salmon (Oncorhynchus Kisutch) by an Immersion-Based Gene Silencing Technology.” Journal of Marine Science and Engineering 11, no. 12 (December 2023): 2208. https://doi.org/10.3390/jmse11122208.
dc.identifier.urihttps://doi.org/10.3390/jmse11122208
dc.identifier.urihttp://hdl.handle.net/11603/30999
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution 4.0 International (CC BY 4.0 DEED)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleInduction of Reproductive Sterility in Coho Salmon (Oncorhynchus kisutch) by an Immersion-Based Gene Silencing Technology
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0002-8978-9233
dcterms.creatorhttps://orcid.org/0000-0002-2756-7790
dcterms.creatorhttps://orcid.org/0000-0001-5573-9767

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