Direct Real Time Detection of Adenosine Triphosphate Release from Astrocytes in Three Dimensional Culture Using an Integrated Electrochemical Aptamer Based Sensor
| dc.contributor.author | Santos-Cancel, Mirelis | |
| dc.contributor.author | Simpson, Laura W. | |
| dc.contributor.author | Leach, Jennie B. | |
| dc.contributor.author | White, Ryan J. | |
| dc.date.accessioned | 2020-08-18T15:29:29Z | |
| dc.date.available | 2020-08-18T15:29:29Z | |
| dc.date.issued | 2019-02-12 | |
| dc.description.abstract | In this manuscript, we describe the development and application of electrochemical aptamer-based (E-AB) sensors directly interfaced with astrocytes in three-dimensional (3D) cell culture to monitor stimulated release of adenosine triphosphate (ATP). The aptamer-based sensor couples specific detection of ATP, selective performance directly in cell culture media, and seconds time resolution using squarewave voltammetry for quantitative ATP-release measurements. More specifically, we demonstrate the ability to quantitatively monitor ATP release into the extracellular environment after stimulation by the addition of calcium (Ca2+), ionomycin, and glutamate. The sensor response is confirmed to be specific to ATP and requires the presence of astrocytes in culture. For example, PC12 cells do not elicit a sensor response after stimulation with the same stimulants. In addition, we confirmed cell viability in the collagen matrix for all conditions tested. Our hydrogel–sensor interface offers the potential to study the release of small molecule messengers in 3D environments. Given the generality of electrochemical aptamer-based sensors and the demonstrated successful interfacing of sensors with tissue scaffold material, in the long term, we anticipate our sensors will be able to translate from in vitro to in vivo small molecule recordings. | en_US |
| dc.description.sponsorship | The authors would like to thank Mr. Danilo Masson de Souza for his contribution and assistance in tissue culture experiments. Funding Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM117159. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health | en_US |
| dc.description.uri | https://pubs.acs.org/doi/10.1021/acschemneuro.9b00033 | en_US |
| dc.format.extent | 24 pages | en_US |
| dc.genre | journal articles postprints | en_US |
| dc.identifier | doi:10.13016/m2inpo-fysm | |
| dc.identifier.citation | Mirelis Santos-Cancel, Laura W. Simpson, Jennie B. Leach, and Ryan J. White, Direct, Real-Time Detection of Adenosine Triphosphate Release from Astrocytes in Three-Dimensional Culture Using an Integrated Electrochemical Aptamer-Based Sensor, ACS Chemical Neuroscience 2019 10 (4), 2070-2079 DOI: 10.1021/acschemneuro.9b00033 | en_US |
| dc.identifier.uri | https://doi.org/10.1021/acschemneuro.9b00033 | |
| dc.identifier.uri | http://hdl.handle.net/11603/19457 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | ACS Publications | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Chemical, Biochemical & Environmental Engineering Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Neuroscience, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acschemneuro.9b00033 | |
| dc.title | Direct Real Time Detection of Adenosine Triphosphate Release from Astrocytes in Three Dimensional Culture Using an Integrated Electrochemical Aptamer Based Sensor | en_US |
| dc.type | Text | en_US |