Extraction and Sensitive Detection of Toxins A and B from the Human Pathogen Clostridium difficile in 40 Seconds Using Microwave-Accelerated Metal-Enhanced Fluorescence

dc.contributor.authorJoshi, Lovleen Tina
dc.contributor.authorMali, Buddha L.
dc.contributor.authorGeddes, Chris
dc.contributor.authorBaillie, Les
dc.date.accessioned2024-10-01T18:05:27Z
dc.date.available2024-10-01T18:05:27Z
dc.date.issued2014-08-27
dc.description.abstractClostridium difficile is the primary cause of antibiotic associated diarrhea in humans and is a significant cause of morbidity and mortality. Thus the rapid and accurate identification of this pathogen in clinical samples, such as feces, is a key step in reducing the devastating impact of this disease. The bacterium produces two toxins, A and B, which are thought to be responsible for the majority of the pathology associated with the disease, although the relative contribution of each is currently a subject of debate. For this reason we have developed a rapid detection assay based on microwave-accelerated metal-enhanced fluorescence which is capable of detecting the presence of 10 bacteria in unprocessed human feces within 40 seconds. These promising results suggest that this prototype biosensor has the potential to be developed into a rapid, point of care, real time diagnostic assay for C. difficile.
dc.description.sponsorshipThis work was supported by: 1. Cardiff University Travel grant to fund travel and work of Lovleen Tina Joshi at the Institute of Fluorescence, Baltimore, USA; 2. Mid-Atlantic Regional Center of Excellence for Biodefence & Emerging Infectious diseases for funding; and 3. NIH/NIAII for funding: grant 2 US4AO57168- 09. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
dc.description.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0104334
dc.format.extent9 pages
dc.genrejournal articles
dc.identifierdoi:10.13016/m2ugdr-6mqw
dc.identifier.citationJoshi, Lovleen Tina, Buddha L. Mali, Chris D. Geddes, and Les Baillie. ‘Extraction and Sensitive Detection of Toxins A and B from the Human Pathogen Clostridium Difficile in 40 Seconds Using Microwave-Accelerated Metal-Enhanced Fluorescence’. PLOS ONE 9, no. 8 (27 August 2014): e104334. https://doi.org/10.1371/journal.pone.0104334.
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0104334
dc.identifier.urihttp://hdl.handle.net/11603/36574
dc.language.isoen_US
dc.publisherPLOS
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Institute of Fluorescence (IoF)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Chemistry & Biochemistry Department
dc.relation.ispartofUMBC Student Collection
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectOligonucleotides
dc.subjectClostridium difficile
dc.subjectMicrowave radiation
dc.subjectFluorescence
dc.subjectGut bacteria
dc.subjectBacterial spores
dc.subjectToxins
dc.subjectPolymerase chain reaction
dc.titleExtraction and Sensitive Detection of Toxins A and B from the Human Pathogen Clostridium difficile in 40 Seconds Using Microwave-Accelerated Metal-Enhanced Fluorescence
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0002-9110-6374

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