Heterogeneous Expression of the Potassium-Chloride Cotransporter KCC₂ in Gonadotropin-Releasing Hormone Neurons of the Adult Mouse

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dc.contributor.authorLeupen, Sarah
dc.contributor.authorTobet, Stuart A.
dc.contributor.authorCrowley, W. F. Jr.,
dc.contributor.authorKaila, Kai
dc.date.accessioned2020-06-22T18:14:58Z
dc.date.available2020-06-22T18:14:58Z
dc.date.issued2003-07-01
dc.description.abstractIn mature central neurons, chloride extrusion mediated by the K-Cl cotransporter KCC₂ appears to be largely responsible for the Cl− driving force that allows γ-aminobutyric acidA (GABAA) receptor activation to trigger a hyperpolarization. In its absence, GABA’s effect is typically depolarizing and often excitatory. We examined the colocalization of KCC₂ and GnRH in adult male and female mice using a combined in situ hybridization-immunofluorescence procedure. We found that KCC₂ was localized to approximately 34% of GnRH neurons. This proportion was similar in females and males. However, females exhibited a marked rostrocaudal gradient of colocalization that was not seen in males. By contrast, KCC₂ was localized to nearly all vasopressin neurons of the supraoptic nucleus. These results indicate that a substantial fraction of GnRH neurons may be depolarized and excited by GABAA receptor activation throughout life, supporting the existence of functionally heterogeneous subpopulations.en_US
dc.description.sponsorshipWe thank Sue Moenter for providing founder mice and continuing discussion; Aline Davis, Heather Walker, and Marianne Seney for technical assistance; and Mark Palmert for helpful discussions.en_US
dc.description.urihttps://academic.oup.com/endo/article/144/7/3031/2888903en_US
dc.format.extent6 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2dsi8-pqy7
dc.identifier.citationSarah M. Leupen, Stuart A. Tobet, W. F. Crowley, Jr., Kai Kaila, Heterogeneous Expression of the Potassium-Chloride Cotransporter KCC₂ in Gonadotropin-Releasing Hormone Neurons of the Adult Mouse, Endocrinology, Volume 144, Issue 7, 1 July 2003, Pages 3031–3036, https://doi.org/10.1210/en.2002-220995en_US
dc.identifier.urihttps://doi.org/10.1210/en.2002-220995
dc.identifier.urihttp://hdl.handle.net/11603/18953
dc.language.isoen_USen_US
dc.publisherOxford Academicen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectNeuroendocrinology
dc.subjectHeterogeneous Expression of the Potassium-Chloride Cotransporter KCC₂ in Gonadotropin
dc.subjectheterogeneous subpopulations
dc.subjectγ-aminobutyric acidᴀ (GᴀBAᴀ)
dc.titleHeterogeneous Expression of the Potassium-Chloride Cotransporter KCC₂ in Gonadotropin-Releasing Hormone Neurons of the Adult Mouseen_US
dc.typeTexten_US

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