Vasoactive intestinal peptide indirectly elicits pituitary LH secretion independent of GnRH in female zebrafish

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dc.contributor.authorTanaka, Sakura
dc.contributor.authorZmora, Nilli
dc.contributor.authorLevavi-Sivan, Berta
dc.contributor.authorZohar, Yonathan
dc.date.accessioned2022-01-21T17:42:13Z
dc.date.available2022-01-21T17:42:13Z
dc.date.issued2022-01-03
dc.description.abstractVasoactive intestinal peptide (Vip) regulates luteinizing hormone (LH) release through the direct regulation of gonadotropin-releasing hormone (GnRH) neurons at the level of the brain in female rodents. However, little is known regarding the roles of Vip in teleost reproduction. Although GnRH is critical for fertility through the regulation of LH secretion in vertebrates, the exact role of the hypophysiotropic GnRH (GnRH3) in zebrafish is unclear since GnRH3 null fish are reproductively fertile. This phenomenon raises the possibility of a redundant regulatory pathway(s) for LH secretion in zebrafish. Here, we demonstrate that VipA (homologues of mammalian Vip) both inhibits and induces LH secretion in zebrafish. Despite the observation that VipA axons may reach the pituitary proximal pars distalis including LH cells, pituitary incubation with VipA in vitro, and intraperitoneal injection of VipA, did not induce LH secretion and lhβ mRNA expression in sexually mature females, respectively. On the other hand, intracerebroventricular administration of VipA augmented plasma LH levels in both wild type and gnrh3-/- females at 1 hour post-treatment, with no observed changes in pituitary GnRH2 and GnRH3 contents and gnrh3 mRNA levels in the brains. While VipA’s manner of inhibition of LH secretion has yet to be explored, the stimulation seems to occur via a different pathway than GnRH3, dopamine, and E2 in regulating LH secretion. The results indicate that VipA induces LH release possibly by acting with or through a non-GnRH factor(s), providing proof for the existence of functional redundancy of LH release in sexually mature female zebrafish.en_US
dc.description.sponsorshipGrants • The U.S. National Science Foundation and the U.S.-Israel Binational Science Foundation Joint Funding Research Grants (# 1947541) • Israel Science Foundation (# 1540/17) • Japan Student Service Organization Study Abroad Graduate Fellowship to S.T. • James Albrecht Graduate Student Fellowship at Institute of Marine and Environmental Technology to S.T.en_US
dc.description.urihttps://academic.oup.com/endo/advance-article-abstract/doi/10.1210/endocr/bqab264/6492622?redirectedFrom=fulltexten_US
dc.format.extent47 pagesen_US
dc.genrejournal articlesen_US
dc.genrepostprintsen_US
dc.identifierdoi:10.13016/m2ofck-jexr
dc.identifier.citationSakura Tanaka, Nilli Zmora, Berta Levavi-Sivan, Yonathan Zohar, Vasoactive intestinal peptide indirectly elicits pituitary LH secretion independent of GnRH in female zebrafish, Endocrinology, 2022;, bqab264, https://doi.org/10.1210/endocr/bqab264en_US
dc.identifier.urihttps://doi.org/10.1210/endocr/bqab264
dc.identifier.urihttp://hdl.handle.net/11603/24052
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis is not the final published version.en_US
dc.rightsAttribution-NonCommercial 4.0 International (CC BY-NC 4.0*
dc.rightsAccess to this item will begin on 1/3/23
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.titleVasoactive intestinal peptide indirectly elicits pituitary LH secretion independent of GnRH in female zebrafishen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0003-3051-6975en_US
dcterms.creatorhttps://orcid.org/0000-0003-1092-3557en_US

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