Advances in zinc sensors for studying zinc release events from pancreatic cells
| dc.contributor.author | Rosenzweig, Zeev | |
| dc.contributor.author | Rosenzweig, Nitsa | |
| dc.contributor.author | Crivat, Georgeta | |
| dc.date.accessioned | 2021-04-19T18:55:27Z | |
| dc.date.available | 2021-04-19T18:55:27Z | |
| dc.date.issued | 2006-10-25 | |
| dc.description | Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 638002 (2006); Event: Optics East 2006, 2006, Boston, Massachusetts, United States | en_US |
| dc.description.abstract | This paper describes the fabrication and characterization of the analytical properties of fluorescence-based zinc ion sensing glass slides and antibody based zinc sensors and their application in monitoring zinc release from beta pancreatic cells. The zinc ion indicator ZnAF-2 {6-[N- [N', N'-bis (2-pyridinylmethyl)-2-aminoethyl] amino-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H] xanthene]-3-one} was modified to include a sufficiently long linking aliphatic chain, with a terminal carboxyl functional group. The activated carboxyl-modified ZnAF-2 was conjugated to the amino silanized surface of glass slides and to free amino groups of the A2B5 antibody molecules. The sensors were used to monitor zinc ion release events from glucose-stimulated pancreatic cells. | en_US |
| dc.description.sponsorship | This work was supported by NSF Grants CHE-0134027. The authors wish to acknowledge the support of Laurie Locascio and Michael Gaitan of NIST and the NIST administration for enabling the completion of this research project at NIST at the aftermath of Hurricane Katrina. | en_US |
| dc.description.uri | https://www.spiedigitallibrary.org/conference-proceedings-of-spie/6380/638002/Advances-in-zinc-sensors-for-studying-zinc-release-events-from/10.1117/12.690653.short?SSO=1 | en_US |
| dc.format.extent | 9 pages | en_US |
| dc.genre | conference papers and proceedings | en_US |
| dc.identifier | doi:10.13016/m2mqto-lzi2 | |
| dc.identifier.citation | Rosenzweig, Zeev; Rosenzweig, Nitsa; Crivat, Georgeta; Advances in zinc sensors for studying zinc release events from pancreatic cells; Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 638002 (2006); https://www.spiedigitallibrary.org/conference-proceedings-of-spie/6380/638002/Advances-in-zinc-sensors-for-studying-zinc-release-events-from/10.1117/12.690653.short?SSO=1 | en_US |
| dc.identifier.uri | https://doi.org/10.1117/12.690653 | |
| dc.identifier.uri | http://hdl.handle.net/11603/21351 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | SPIE | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Chemistry & Biochemistry Department Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | ©2006 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited | |
| dc.title | Advances in zinc sensors for studying zinc release events from pancreatic cells | en_US |
| dc.type | Text | en_US |