Optimizing the Separation of an Antiparasitic Medication using High- Pressure Liquid Chromatography (HPLC)

dc.contributor.authorBarnett, Karis R.
dc.contributor.authorLaCourse, William
dc.date.accessioned2024-05-13T19:11:15Z
dc.date.available2024-05-13T19:11:15Z
dc.date.issued2020-12
dc.description.abstractExcess pharmaceutical waste in water is an emerging concern that can increase parasitic drug resistance, interrupt animal food chains, and threaten drinking water sources. In this work, a high-pressure liquid chromatography (HPLC) method with ultraviolet detection (210 nm) was optimized for sensitively detecting and separating antiparasitic compounds praziquantel (PZQ) and metronidazole (MET). This method has the potential to commercially monitor antiparasitic treatments administered to aquatic species, which can ultimately prevent pharmaceutical waste in water. The latest HPLC method was altered over seven experiment trials to improve resolution and Gaussian shape of chromatogram peaks. The most efficient separation of PZQ and MET was achieved on a Phenomenex™ Luna C18 analytical column (150 x 4.60mm, 5μm, 100A) using acetonitrile:water at alternating ratios of 20:80 v/v and 80:20 v/v as a mobile phase. This separation resulted in the shortest acquisition time with satisfactory peak shape. Aquarium facilities may ultimately use this method to understand how to safely treat parasitic fish diseases while avoiding environmental damage.
dc.description.urihttps://provost.utsa.edu/undergraduate-research/journal/files/vol7/JURSW.v7.06.Barnett-LaCourse.pdf
dc.format.extent13 pages
dc.genrejournal articles
dc.identifierdoi:10.13016/m2vjyf-xqie
dc.identifier.citationBarnett, Karis R, and William R LaCourse. “Optimizing the Separation of an Antiparasitic Medication Using High- Pressure Liquid Chromatography (HPLC)” UTSA Journal of Undergraduate Research and Scholarly Works 7 (Decemeber 2020). https://provost.utsa.edu/undergraduate-research/journal/files/vol7/JURSW.v7.06.Barnett-LaCourse.pdf
dc.identifier.urihttp://hdl.handle.net/11603/33945
dc.language.isoen_US
dc.publisherUTSA Office of Undergraduate Research
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Office of the Dean of the College of Natural and Mathematical Sciences
dc.relation.ispartofUMBC Chemistry & Biochemistry Department
dc.relation.ispartofUMBC Student Collection
dc.subjectHPLC
dc.subjectantiparasitics
dc.subjectseparations
dc.subjectaquatic environment
dc.titleOptimizing the Separation of an Antiparasitic Medication using High- Pressure Liquid Chromatography (HPLC)
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0003-2635-9460

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