Cell deformation cytometry using diode-bar optical stretchers

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dc.contributor.authorSraj, Ihab
dc.contributor.authorEggleton, Charles D.
dc.contributor.authorJimenez, Ralph
dc.contributor.authorHoover, Erich E.
dc.contributor.authorSquier, Jeffrey A.
dc.contributor.authorChichester, Justin
dc.contributor.authorMarr, David W.
dc.date.accessioned2018-10-22T13:27:38Z
dc.date.available2018-10-22T13:27:38Z
dc.date.issued2010-08-04
dc.description.abstractThe measurement of cell elastic parameters using optical forces has great potential as a reagent-free method for cell classification, identification of phenotype, and detection of disease; however, the low throughput associated with the sequential isolation and probing of individual cells has significantly limited its utility and application. We demonstrate a single-beam, high-throughput method where optical forces are applied anisotropically to stretch swollen erythrocytes in microfluidic flow. We also present numerical simulations of model spherical elastic cells subjected to optical forces and show that dual, opposing optical traps are not required and that even a single linear trap can induce cell stretching, greatly simplifying experimental implementation. Last, we demonstrate how the elastic modulus of the cell can be determined from experimental measurements of the equilibrium deformation. This new optical approach has the potential to be readily integrated with other cytometric technologies and, with the capability of measuring cell populations, enabling true mechanical-property-based cell cytometry.en_US
dc.description.sponsorshipThe authors gratefully acknowledge support from the National Science Foundation under Grant No. DBI-045468, the National Institutes of Health under Grant No. RO1-AI063366, and from the Butcher Foundation. RJ is a staff member in the Quantum Physics Division of NIST.en_US
dc.description.urihttps://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-15/issue-04/047010/Cell-deformation-cytometry-using-diode-bar-optical-stretchers/10.1117/1.3470124.full?SSO=1en_US
dc.format.extent7 pagesen_US
dc.genrejournal articleen_US
dc.identifierdoi:10.13016/M2ZS2KH4Q
dc.identifier.citationIhab Sraj, Charles D. Eggleton, Ralph Jimenez, Erich E. Hoover, Jeffrey A. Squier, Justin Chichester, David W. Marr , Cell deformation cytometry using diode-bar optical stretchers , J. of Biomedical Optics, 15(4), 047010 (2010). https://doi.org/10.1117/1.3470124en_US
dc.identifier.urihttps://doi.org/10.1117/1.3470124
dc.identifier.urihttp://hdl.handle.net/11603/11625
dc.language.isoen_USen_US
dc.publisherSociety of Photo-Optical Instrumentation Engineers.en_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Mechanical Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rights© Copyright 2010 International Society of Optics and Photonics
dc.subjectbiomedical opticsen_US
dc.subjectcellsen_US
dc.subjectdetectorsen_US
dc.subjectUMBC High Performance Computing Facility (HPCF)en_US
dc.titleCell deformation cytometry using diode-bar optical stretchersen_US
dc.typeTexten_US

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