Malic Acid Carbon Dots: From Super-resolution Live-Cell Imaging to Highly Efficient Separation

Zhi, Bo; Cui, Yi; Wang, Shengyang; Frank, Benjamin P.; Williams, Denise N.; Brown, Richard; Melby, Eric S.; Hamers, Robert J.; Rosenzweig, Zeev; Fairbrother, D. Howard; Orr, Galya; Haynes, Christy L.

Malic Acid Carbon Dots: From Super-resolution Live-Cell Imaging to Highly Efficient Separation

dc.contributor.author	Zhi, Bo
dc.contributor.author	Cui, Yi
dc.contributor.author	Wang, Shengyang
dc.contributor.author	Frank, Benjamin P.
dc.contributor.author	Williams, Denise N.
dc.contributor.author	Brown, Richard
dc.contributor.author	Melby, Eric S.
dc.contributor.author	Hamers, Robert J.
dc.contributor.author	Rosenzweig, Zeev
dc.contributor.author	Fairbrother, D. Howard
dc.contributor.author	Orr, Galya
dc.contributor.author	Haynes, Christy L.
dc.date.accessioned	2021-04-14T17:21:29Z
dc.date.available	2021-04-14T17:21:29Z
dc.date.issued	2018-06-08
dc.description.abstract	As-synthesized malic acid carbon dots are found to possess photoblinking properties that are outstanding and superior compared to those of conventional dyes. Considering their excellent biocompatibility, malic acid carbon dots are suitable for super-resolution fluorescence localization microscopy under a variety of conditions, as we demonstrate in fixed and live trout gill epithelial cells. In addition, during imaging experiments, the so-called “excitation wavelength-dependent” emission was not observed for individual as-made malic acid carbon dots, which motivated us to develop a time-saving and high-throughput separation technique to isolate malic acid carbon dots into fractions of different particle size distributions using C18 reversed-phase silica gel column chromatography. This post-treatment allowed us to determine how particle size distribution influences the optical properties of malic acid carbon dot fractions, that is, optical band gap energies and photoluminescence behaviors.	en_US
dc.description.sponsorship	This work was supported by the National Science Foundation Center for Chemical Innovation Program Grant No. CHE1503408 under the Center for Sustainable Nanotechnology. We thank Dr. Christopher J. Douglas (University of Minnesota) for carbon dot separation supplies and equipment. Part of this work, including super-resolution cell imaging and fluorescence characterization of single dots, was performed using the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy’s Office of Biological and Environmental Research and located at Pacific Northwest National Laboratory. Parts of this work, including XPS and TEM characterization, were carried out in the Characterization Facility, University of Minnesota, which receives partial support from the MRSEC program (DMR-1420013).	en_US
dc.description.uri	https://pubs.acs.org/doi/10.1021/acsnano.8b01619	en_US
dc.format.extent	6 files	en_US
dc.genre	journal articles	en_US
dc.identifier	doi:10.13016/m2ge8g-vs9z
dc.identifier.citation	Zhi, Bo; Cui, Yi; Wang, Shengyang; Frank, Benjamin P.; Williams, Denise N.; Brown, Richard; Melby, Eric S.; Hamers, Robert J.; Rosenzweig, Zeev; Fairbrother, D. Howard; Orr, Galya; Haynes, Christy L.; Malic Acid Carbon Dots: From Super-resolution Live-Cell Imaging to Highly Efficient Separation; ACS Nano 12, 6, 5741–5752 (2018); https://pubs.acs.org/doi/10.1021/acsnano.8b01619	en_US
dc.identifier.uri	https://doi.org/10.1021/acsnano.8b01619
dc.identifier.uri	http://hdl.handle.net/11603/21334
dc.language.iso	en_US	en_US
dc.publisher	ACS Publications	en_US
dc.relation.isAvailableAt	The University of Maryland, Baltimore County (UMBC)
dc.relation.ispartof	UMBC Chemistry & Biochemistry Department Collection
dc.relation.ispartof	UMBC Faculty Collection
dc.relation.ispartof	UMBC Student Collection
dc.rights	This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rights	Public Domain Mark 1.0	*
dc.rights	This work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.
dc.rights.uri	http://creativecommons.org/publicdomain/mark/1.0/	*
dc.title	Malic Acid Carbon Dots: From Super-resolution Live-Cell Imaging to Highly Efficient Separation	en_US
dc.type	Text	en_US

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