Secondary structure of the leader transcript from the Escherichia coli S10 ribosomal protein operon

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dc.contributor.authorShen, P.
dc.contributor.authorZengel, J. M.
dc.contributor.authorLindahl, L.
dc.date.accessioned2019-06-05T17:47:29Z
dc.date.available2019-06-05T17:47:29Z
dc.date.issued1988-09-26
dc.description.abstractGenetic analysis of the autogenous control of the S10 ribosomal protein operon of Escherichia coli has suggested that the secondary or tertiary structure of the leader transcript is important for this regulation. We have therefore determined the secondary structure of the leader by enzyme digestion and chemical modification. Our results suggest that the 172 base leader exists in two forms, differing only immediately upstream of the Shine-Dalgarno sequence of the first gene. We discuss the possibility that the equilibrium between these alternate structures is important for the L4-mediated regulation of translation of the S10 operon. We have also determined the structure of several mutant transcripts. Correlation of these structures with the regulatory phenotypes suggest that a hairpin about 50 bases upstream of the first gene is essential for the control of translation of the operon. Finally, our results show that a two base substitution in an eight base loop destabilizes the attached stem.en_US
dc.description.sponsorshipThis work was supported by a research grant and a Research Career Development Award to LL from the National Institute for Allergy and Infectious Diseases.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC338642/en_US
dc.format.extent20 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2mejo-kvv4
dc.identifier.citationP Shen, J M Zengel, and L Lindahl, Secondary structure of the leader transcript from the Escherichia coli S10 ribosomal protein operon, Nucleic Acids Res. 1988 Sep 26; 16(18): 8905–8924. doi: 10.1093/nar/16.18.8905en_US
dc.identifier.urihttps://dx.doi.org/10.1093%2Fnar%2F16.18.8905
dc.identifier.urihttp://hdl.handle.net/11603/14020
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectribosomal protein operonen_US
dc.subjectEscherichia colien_US
dc.subjectenzyme digestionen_US
dc.titleSecondary structure of the leader transcript from the Escherichia coli S10 ribosomal protein operonen_US
dc.typeTexten_US

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