β-Glutamate as a Substrate for Glutamine Synthetase

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dc.contributor.authorRobinson, Patrice
dc.contributor.authorNeelon, Kelly
dc.contributor.authorSchreier, Harold J.
dc.contributor.authorRoberts, Mary F.
dc.date.accessioned2019-03-20T14:42:31Z
dc.date.available2019-03-20T14:42:31Z
dc.date.issued2001-10
dc.description.abstractThe conversion of β-glutamate to β-glutamine by archaeal and bacterial glutamine synthetase (GS) enzymes has been examined. The GS from Methanohalophilus portucalensis (which was partially purified) is capable of catalyzing the amidation of this substrate with a rate sevenfold less than the rate obtained with α-glutamate. Recombinant GS from the archaea Methanococcus jannaschii and Archaeoglobus fulgidus were considerably more selective for α-glutamate than β-glutamate as a substrate. All the archaeal enzymes were much less selective than the two bacterial GS (from Escherichia coli and Bacillus subtilis), whose specific activities towards β-glutamate were much smaller than rates with the α-isomer. These results are discussed in light of the observation that β-glutamate is accumulated as an osmolyte in many archaea while β-glutamine (produced by glutamine synthetase) is used as an osmolyte only in M. portucalensis.en_US
dc.description.sponsorshipThis work has been supported, in part, by grant DE-FG02-91ER20025 (to M.F.R.) from the Department of Energy Biosciences Division and by a grant from the National Science Foundation (H.J.S.) and the Wallenburg Foundation VIRTUE program (H.J.S.).en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC93190/en_US
dc.format.extent6 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2zasc-fhph
dc.identifier.citationPatrice Robinson, Kelly Neelon, Harold J. Schreier, and Mary F. Roberts, β-Glutamate as a Substrate for Glutamine Synthetase, Appl Environ Microbiol. 2001 Oct; 67(10): 4458–4463. doi: 10.1128/AEM.67.10.4458-4463.2001en_US
dc.identifier.urihttps://doi.org/10.1128/AEM.67.10.4458-4463.2001
dc.identifier.urihttp://hdl.handle.net/11603/13079
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectMethanohalophilus portucalensisen_US
dc.subjectβ-glutamateen_US
dc.subjectbacterial glutamine synthetase (GS) enzymesen_US
dc.titleβ-Glutamate as a Substrate for Glutamine Synthetaseen_US
dc.title.alternativebeta-Glutamate as a substrate for glutamine synthetaseen_US
dc.typeTexten_US

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