Rapid recombinant protein expression in cell-free extracts from human blood

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dc.contributor.authorBurgenson, David
dc.contributor.authorGurramkonda, Chandrasekhar
dc.contributor.authorPilli, Manohar
dc.contributor.authorGe, Xudong
dc.contributor.authorAndar, Abhay
dc.contributor.authorKostov, Yordan
dc.contributor.authorTolosa, Leah
dc.contributor.authorRao, Govind
dc.date.accessioned2018-09-10T20:14:43Z
dc.date.available2018-09-10T20:14:43Z
dc.date.issued2018-06-22
dc.description.abstractSeveral groups have recently reported on the utility of cell-free expression systems to make therapeutic proteins, most of them employing CHO or E. coli cell-free extracts. Here, we propose an alternative that uses human blood derived leukocyte cell extracts for the expression of recombinant proteins. We demonstrate expression of nano luciferase (Nluc), Granulocyte-colony stimulating factor (G-CSF) and Erythropoietin (EPO) in cell-free leukocyte extracts within two hours. Human blood is readily available from donors and blood banks and leukocyte rich fractions are easy to obtain. The method described here demonstrates the ability to rapidly express recombinant proteins from human cell extracts that could provide the research community with a facile technology to make their target protein. Eventually, we envision that any recombinant protein can be produced from patient-supplied leukocytes, which can then be injected back into the patient. This approach could lead to an alternative model for personalized medicines and vaccines.en_US
dc.description.sponsorshipThe authors thank the Office of Technology Development, University of Maryland Baltimore County for funding, Nicole Draghic for editorial assistance, and Mike Tolosa for assistance with the illustrations. This paper is dedicated to the late Dr. Michael K. Hooker, former President of UMBC and a philosopher who often spoke about the transformational power of biotechnology for the benefit of mankind.en_US
dc.description.urihttps://www.nature.com/articles/s41598-018-27846-8.pdfen_US
dc.format.extent7 pagesen_US
dc.genrejournal articleen_US
dc.identifierdoi:10.13016/M2N87334G
dc.identifier.citationDavid Burgenson, Chandrasekhar Gurramkonda , Manohar Pilli, Xudong Ge, Abhay Andar, Yordan Kostov, Leah Tolosa, Govind Rao, Rapid recombinant protein expression in cell-free extracts from human blood, Scientific Reports, 2018, https://doi.org/10.1038/s41598-018-27846-8en_US
dc.identifier.uri10.1038/s41598-018-27846-8 ID
dc.identifier.urihttp://hdl.handle.net/11603/11273
dc.language.isoen_USen_US
dc.publisherNatureen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Center for Advanced Photonics Rsearch (CASPR)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjecttherapeutic proteinsen_US
dc.subjectnano luciferase (Nluc)en_US
dc.subjectGranulocyte-colony stimulating factor (G-CSF)en_US
dc.subjectErythropoietin (EPO)en_US
dc.titleRapid recombinant protein expression in cell-free extracts from human blooden_US
dc.typeTexten_US

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