Operon-specific regulation of ribosomal protein synthesis in Escherichia coli

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dc.contributor.authorLindahl, L.
dc.contributor.authorZengel, J. M.
dc.date.accessioned2019-06-04T17:59:43Z
dc.date.available2019-06-04T17:59:43Z
dc.date.issued1979-12
dc.description.abstractWe have cloned a DNA fragment harboring the genes for ribosomal proteins L2, L4, and L23 on a plasmid vector that contains a lac operator and promoter. The cloned ribosomal protein genes are now under the control of lacOP. Addition of a lac inducer to these cells results in a specific 5- to 10-fold increase in the synthesis of the proteins corresponding to the cloned genes. Within 10 min of this induction, the synthesis of ribosomal proteins S3, S19, L3, L16, L22, and L29 stops almost completely. The genes for all these proteins reside in the same chromosomal operon as L2, L4, and L23. We have seen no dramatic effect on the synthesis of any other ribosomal proteins. Thus, the induction of L2, L4, and L23 results in a specific and rapid decrease in the expression of all (or almost all) genes in their own transcription unit.en_US
dc.description.sponsorshipThis work was supported by grants from the National Institute for Allergy and Infectious Diseases and the New York State Health Research Council.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC411902/en_US
dc.format.extent5 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2hkfd-ieqe
dc.identifier.citationL. Lindahl and J. M. Zengel, Operon-specific regulation of ribosomal protein synthesis in Escherichia coli, Proc Natl Acad Sci U S A. 1979 Dec; 76(12): 6542–6546. doi: 10.1073/pnas.76.12.6542en_US
dc.identifier.urihttps://dx.doi.org/10.1073%2Fpnas.76.12.6542
dc.identifier.urihttp://hdl.handle.net/11603/14010
dc.language.isoen_USen_US
dc.publisherNational Academy of Sciencesen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectribosomal proteinsen_US
dc.subjectisopropylthiogalactoside (IPTG)en_US
dc.subjectEscherichia colien_US
dc.titleOperon-specific regulation of ribosomal protein synthesis in Escherichia colien_US
dc.typeTexten_US

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