Chemogenetic Depletion of Hypophysiotropic GnRH Neurons Does Not Affect Fertility in Mature Female Zebrafish

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dc.contributor.authorTanaka, Sakura
dc.contributor.authorZmora, Nilli
dc.contributor.authorLevavi-Sivan, Berta
dc.contributor.authorZohar, Yonathan
dc.date.accessioned2022-06-23T21:13:10Z
dc.date.available2022-06-23T21:13:10Z
dc.date.issued2022-05-17
dc.description.abstractThe hypophysiotropic gonadotropin-releasing hormone (GnRH) and its neurons are crucial for vertebrate reproduction, primarily in regulating luteinizing hormone (LH) secretion and ovulation. However, in zebrafish, which lack GnRH1, and instead possess GnRH3 as the hypophysiotropic form, GnRH3 gene knockout did not affect reproduction. However, early-stage ablation of all GnRH3 neurons causes infertility in females, implicating GnRH3 neurons, rather than GnRH3 peptides in female reproduction. To determine the role of GnRH3 neurons in the reproduction of adult females, a Tg(gnrh3:Gal4ff; UAS:nfsb-mCherry) line was generated to facilitate a chemogenetic conditional ablation of GnRH3 neurons. Following ablation, there was a reduction of preoptic area GnRH3 neurons by an average of 85.3%, which was associated with reduced pituitary projections and gnrh3 mRNA levels. However, plasma LH levels were unaffected, and the ablated females displayed normal reproductive capacity. There was no correlation between the number of remaining GnRH3 neurons and reproductive performance. Though it is possible that the few remaining GnRH3 neurons can still induce an LH surge, our findings are consistent with the idea that GnRH and its neurons are likely dispensable for LH surge in zebrafish. Altogether, our results resurrected questions regarding the functional homology of the hypophysiotropic GnRH1 and GnRH3 in controlling ovulation.en_US
dc.description.sponsorshipThis research was funded by the U.S. National Science Foundation and the U.S.-Israel Binational Science Foundation Joint Funding Research Grants (# 947541) and Israel Science Foundation (# 1540/17).en_US
dc.description.urihttps://www.mdpi.com/1422-0067/23/10/5596en_US
dc.format.extent19 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2wlox-qjrd
dc.identifier.citationTanaka, Sakura, Nilli Zmora, Berta Levavi-Sivan, and Yonathan Zohar. 2022. "Chemogenetic Depletion of Hypophysiotropic GnRH Neurons Does Not Affect Fertility in Mature Female Zebrafish" International Journal of Molecular Sciences 23, no. 10: 5596. https://doi.org/10.3390/ijms23105596en_US
dc.identifier.urihttps://doi.org/10.3390/ijms23105596
dc.identifier.urihttp://hdl.handle.net/11603/25032
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleChemogenetic Depletion of Hypophysiotropic GnRH Neurons Does Not Affect Fertility in Mature Female Zebrafishen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0003-3051-6975en_US
dcterms.creatorhttps://orcid.org/0000-0003-1324-2458en_US

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