Mild Whole Body Hyperthermia Induced Interstitial Fluid Pressure (IFP) Reduction and Enhanced Nanoparticle Delivery to PC3 Tumors: In Vivo Studies and MicroCT Analyses

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dc.contributor.authorGu, Qimei
dc.contributor.authorLiu, Shuaishuai
dc.contributor.authorRay, Arunendra Saha
dc.contributor.authorFlorinas, Stelios
dc.contributor.authorChristie, Ronald James
dc.contributor.authorDaniel, Marie-Christine
dc.contributor.authorBieberich, Charles
dc.contributor.authorMa, Ronghui
dc.contributor.authorZhu, Liang
dc.date.accessioned2020-04-10T19:04:10Z
dc.date.available2020-04-10T19:04:10Z
dc.date.issued2020-02-28
dc.description.abstractIn vivo experiments on mice were performed to evaluate whether whole body hyperthermia enhances nanoparticle delivery to PC3 tumors. The mice in the experimental group were subjected to whole body hyperthermia by maintaining their body temperatures at 40ºC for one hour. Interstitial fluid pressures (IFPs) in tumors were measured before heating, immediately after, and at 2 and 24 hours post-heating in both the experimental group and in a sham group (without heating). 0.2 cc of a newly developed nanofluid containing gold nanoparticles (AuNPs) was delivered via the tail vein in both groups. MicroCT scanned images of resected tumors were analyzed to visualize nanoparticle distribution in the tumors and to quantify the total amount of the nanoparticles delivered to the tumors. Statistically significant IFP reductions of 45% right after heating, 47% 2 hours post heating, and 52% 24 hours post heating were observed in the experimental group. Analyses of microCT scans of the resected tumors illustrated that nanoparticles were more concentrated near the tumor periphery rather than at the tumor center. The 1-hour whole body hyperthermia treatment resulted in more nanoparticles present in the tumor central region than that in the control group. The mass index calculated from the microCT scans suggested overall 42% more nanoparticle delivery in the experimental group than that in the control group. We conclude that 1-hour mild whole body hyperthermia leads to sustained reduction in tumoral IFPs and significantly increases the total amount of targeted gold nanoparticle deposition in PC3 tumors.en_US
dc.description.sponsorshipThis research was supported by an NSF research grant (CBET-1705538). The research was performed in partial fulfillment of the requirements for the PhD degree by Qimei Gu from the University of Maryland Baltimore County, Baltimore, Maryland, USA. The authors are also thankful to Tagide deCarvalho for her assistance in sample preparation and acquisition of TEM images, and Ryan Fleming for expression and purification of the anti-EphA2 Fab protein.en_US
dc.description.urihttps://asmedigitalcollection.asme.org/thermalscienceapplication/article-abstract/doi/10.1115/1.4046520/1074975/Mild-Whole-Body-Hyperthermia-Induced-Interstitial?redirectedFrom=fulltexten_US
dc.format.extent35 pagesen_US
dc.genrejournal articles preprintsen_US
dc.identifierdoi:10.13016/m2qznn-t7yh
dc.identifier.citationGu, Qimei; Liu, Shuaishuai; Ray, Arunendra Saha; Florinas, Stelios; Christie, Ronald James; Daniel, Marie-Christine; Bieberich, Charles; Ma, Ronghui; Zhu, Liang; Mild Whole Body Hyperthermia Induced Interstitial Fluid Pressure (IFP) Reduction and Enhanced Nanoparticle Delivery to PC3 Tumors: In Vivo Studies and MicroCT Analyses; JOurnal of Thermal Science and Engineering Applications (1-23) (2020); https://asmedigitalcollection.asme.org/thermalscienceapplication/article-abstract/doi/10.1115/1.4046520/1074975/Mild-Whole-Body-Hyperthermia-Induced-Interstitial?redirectedFrom=fulltexten_US
dc.identifier.urihttps://doi.org/10.1115/1.4046520
dc.identifier.urihttp://hdl.handle.net/11603/17988
dc.language.isoen_USen_US
dc.publisherThe American Society of Mechanical Engineersen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Mechanical Engineering Department Collection
dc.relation.ispartofUMBC Biological Sciences Department
dc.relation.ispartofUMBC Chemistry & Biochemistry Department
dc.relation.ispartofUMBC Student Collection
dc.relation.ispartofUMBC Faculty Collection
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dc.rightsAuthors may reproduce and distribute the Paper for non-commercial purposes only. Non-commercial applies only to the sale of the paper per se. For all copies of the Paper made by Authors, Authors must acknowledge ASME as original publisher and include the names of all author(s), the publication title, and an appropriate copyright notice that identifies ASME as the copyright holder.
dc.rightsAccess to this item will begin on 2021-02-28
dc.titleMild Whole Body Hyperthermia Induced Interstitial Fluid Pressure (IFP) Reduction and Enhanced Nanoparticle Delivery to PC3 Tumors: In Vivo Studies and MicroCT Analysesen_US
dc.typeTexten_US

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