Phylogenetic analysis of the structure of RNase MRP RNA in yeasts

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dc.contributor.authorLi, Xing
dc.contributor.authorFrank, Daniel N.
dc.contributor.authorPace, Norman
dc.contributor.authorZengel, Janice M.
dc.contributor.authorLindahl, Lasse
dc.date.accessioned2019-06-19T17:20:34Z
dc.date.available2019-06-19T17:20:34Z
dc.date.issued2002-06
dc.description.abstractRNase MRP is a ribonucleoprotein enzyme involved in processing precursor rRNA in eukaryotes. To facilitate our structure-function analysis of RNase MRP from Saccharomyces cerevisiae, we have determined the likely secondary structure of the RNA component by a phylogenetic approach in which we sequenced all or part of the RNase MRP RNAs from 17 additional species of the Saccharomycetaceae family. The structure deduced from these sequences contains the helices previously suggested to be common to the RNA subunit of RNase MRP and the related RNA subunit of RNase P, an enzyme cleaving tRNA precursors. However, outside this common region, the structure of RNase MRP RNA determined here differs from a previously proposed universal structure for RNase MRPs. Chemical and enzymatic structure probing analyses were consistent with our revised secondary structure. Comparison of all known RNase MRP RNA sequences revealed three regions with highly conserved nucleotides. Two of these regions are part of a helix implicated in RNA catalysis in RNase P, suggesting that RNase MRP may cleave rRNA using a similar catalytic mechanism.en_US
dc.description.sponsorshipThis work was supported by Grant MCB 0077949 from the National Science Foundation to L.L. and J.Z., and Grant GM 34527 from the National Institutes of Health to N.P.en_US
dc.description.urihttps://www.cambridge.org/core/journals/rna/article/phylogenetic-analysis-of-the-structure-of-rnase-mrp-rna-in-yeasts/95586A36642573C5D04AE339991E9599en_US
dc.format.extent12 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2o6vr-opga
dc.identifier.citationXing Li, et.al, Phylogenetic analysis of the structure of RNase MRP RNA in yeasts, RNA. 2002 Jun; 8(6): 740–751, DOI: https://doi.org/10.1017/s1355838202022082en_US
dc.identifier.urihttps://doi.org/10.1017/s1355838202022082
dc.identifier.urihttp://hdl.handle.net/11603/14270
dc.language.isoen_USen_US
dc.publisherThe RNA Societyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectRNA catalysisen_US
dc.subjectRNA processingen_US
dc.subjectrRNAen_US
dc.titlePhylogenetic analysis of the structure of RNase MRP RNA in yeastsen_US
dc.typeTexten_US

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