Differences in LexA regulon structure among Proteobacteria through in vivo assisted comparative genomics
No Thumbnail Available
Links to Files
Citation of Original Publication
Ivan Erill, Mónica Jara, Noelia Salvador, Marcos Escribano, Susana Campoy, Jordi Barbé, Differences in LexA regulon structure among Proteobacteria through in vivo assisted comparative genomics , Nucleic Acids Research, Volume 32, Issue 22, 15 November 2004, Pages 6617–6626, https://doi.org/10.1093/nar/gkh996
This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
The LexA regulon encompasses an ensemble of genes involved in preserving cell viability under massive DNA damage and is present in most bacterial phyla. Up to date, however, the scope of this network had only been assessed in the Gamma Proteobacteria. Here, we report the structure of the LexA regulon in the Alpha Proteobacteria, using a combined approach that makes use of in vitro and in vivo techniques to assist and validate the comparative genomics in silico methodology. This leads to the first experimentally validated description of the LexA regulon in the Alpha Proteobacteria, and comparison of regulon core structures in both classes suggests that a least common multiple set of genes ( recA , ssb , uvrA and ruvCAB ) might be a defining property of the Proteobacteria LexA network.