A macroporous hydrogel for the coculture of neural progenitor and endothelial cells to form functional vascular networks invivo

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dc.contributor.authorFord, Millicent C.
dc.contributor.authorBertram, James P.
dc.contributor.authorHynes, Sara Royce
dc.contributor.authorMichaud, Michael
dc.contributor.authorLi, Qi
dc.contributor.authorYoung, Michael
dc.contributor.authorSegal, Steven S.
dc.contributor.authorMadri, Joseph A.
dc.contributor.authorLavik, Erin
dc.date.accessioned2021-03-19T17:38:48Z
dc.date.available2021-03-19T17:38:48Z
dc.date.issued2006-02-21
dc.descriptionProceedings of the National Academy of Sciences Feb 2006, 103 (8) 2512-2517;en_US
dc.description.abstractA microvascular network is critical for the survival and function of most tissues. We have investigated the potential of neural progenitor cells to augment the formation and stabilization of microvascular networks in a previously uncharacterized three-dimensional macroporous hydrogel and the ability of this engineered system to develop a functional microcirculation in vivo. The hydrogel is synthesized by cross-linking polyethylene glycol with polylysine around a salt-leached polylactic-co-glycolic acid scaffold that is degraded in a sodium hydroxide solution. An open macroporous network is formed that supports the efficient formation of tubular structures by brain endothelial cells. After subcutaneous implantation of hydrogel cocultures in mice, blood flow in new microvessels was apparent at 2 weeks with perfused networks established on the surface of implants at 6 weeks. Compared to endothelial cells cultured alone, cocultures of endothelial cells and neural progenitor cells had a significantly greater density of tubular structures positive for platelet endothelial cell adhesion molecule-1 at the 6-week time point. In implant cross sections, the presence of red blood cells in vessel lumens confirmed a functional microcirculation. These findings indicate that neural progenitor cells promote the formation of endothelial cell tubes in coculture and the development of a functional microcirculation in vivo. We demonstrate a previously undescribed strategy for creating stable microvascular networks to support engineered tissues of desired parenchymal cell origin.en_US
dc.description.sponsorshipWe thank Justin Roh and Rebecca Royce for all of their assistance with histology. This work was funded by a generous gift from Richard and Gail Siegal as well as National Institutes of Health (NIH) Grant P01-NS35476 (to J.A.M.). S.R.H. acknowledges National Science Foundation GK-12 Teaching Fellowship DGE-0231832, and M.C.F. and J.P.B. acknowledge NIH Neuroengineering Training Grant T90-DK070068.en_US
dc.description.urihttps://www.pnas.org/content/103/8/2512.shorten_US
dc.format.extent6 pagesen_US
dc.genreconference papers and proceedingsen_US
dc.identifierdoi:10.13016/m2kx6v-iyxl
dc.identifier.citationFord et al., A macroporous hydrogel for the coculture of neural progenitor and endothelial cells to form functional vascular networks invivo, PNAS, 103 (8) 2512-2517; https://doi.org/10.1073/pnas.0506020102en_US
dc.identifier.urihttps://doi.org/10.1073/pnas.0506020102
dc.identifier.urihttp://hdl.handle.net/11603/21197
dc.language.isoen_USen_US
dc.publisherPNASen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectmicrovasculatureen_US
dc.subjectneural stem cellsen_US
dc.subjectpolymeren_US
dc.subjectscaffolden_US
dc.titleA macroporous hydrogel for the coculture of neural progenitor and endothelial cells to form functional vascular networks invivoen_US
dc.typeTexten_US

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